A surface plasmon resonance (SPR) sensor for detection of toluene (C6H5CH3)

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dc.contributor.authorT T Dung-
dc.contributor.authorMoonil Kim-
dc.date.accessioned2019-01-23T16:30:53Z-
dc.date.available2019-01-23T16:30:53Z-
dc.date.issued2018-
dc.identifier.issn22886559-
dc.identifier.uri10.5757/ASCT.2018.27.6.184ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/18234-
dc.description.abstractHerein, we report a novel surface plasmon resonance (SPR) sensor modified with toluene binding domain (TBD) to detect toluene. The toluene binding domain was manipulated with cysteines for rapid, easy and orientation-controlled immobilization of the protein onto a SPR gold surface due to the specific interaction between a bare gold and the thiol group (-SH) of cysteines. After the purification and on-chip immobilization of the recombinant TBD, the thickness of cysteine-tagged TBD was determined to be approximately 1.8 nm according to the cross-sectional analysis by atomic force microscopy (AFM). The SPR analysis of cysteine-tagged TBD adsorption showed that cysteine-tagged TBD bound onto a gold thin film with high binding affinity. With a SPR-based ligand binding assay, a detectable increase in the SPR response was observed with 15.62 uM of toluene. Due to the inherent specificity of TBD, this method showed a comparative specificity for the determination of toluene, as it exhibited no SPR response to aromatic compounds such as p-xylene and benzene. Taken together, the results obtained from this study indicate that TBD-modified SPR sensor system can be useful for detecting toluene, a volatile organic compound widely distributed in the environment.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleA surface plasmon resonance (SPR) sensor for detection of toluene (C6H5CH3)-
dc.title.alternativeA surface plasmon resonance (SPR) sensor for detection of toluene (C6H5CH3)-
dc.typeArticle-
dc.citation.titleApplied Science and Convergence Technology-
dc.citation.number6-
dc.citation.endPage188-
dc.citation.startPage184-
dc.citation.volume27-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.alternativeNameDung-
dc.contributor.alternativeName김문일-
dc.identifier.bibliographicCitationApplied Science and Convergence Technology, vol. 27, no. 6, pp. 184-188-
dc.identifier.doi10.5757/ASCT.2018.27.6.184-
dc.subject.keywordBiosensor-
dc.subject.keywordSurface plasmon resonance-
dc.subject.keywordSPR-
dc.subject.keywordToluene-
dc.subject.keywordToluene binding domain-
dc.subject.localBiosensor-
dc.subject.localSurface plasmon resonance-
dc.subject.localSurface plasmon resonance (SPR)-
dc.subject.localSPR-
dc.subject.localToluene-
dc.subject.localToluene binding domain-
dc.description.journalClassN-
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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