Transcriptome profiling and characterization of drought-tolerant potato plant (Solanum tuberosum L.)

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Title
Transcriptome profiling and characterization of drought-tolerant potato plant (Solanum tuberosum L.)
Author(s)
Ki-Beom Moon; Dong Joo Ahn; Ji-Sun Park; Won Yong Jung; Hye Sun ChoHyeran KimJae Heung Jeon; Y I Park; Hyun Soon Kim
Bibliographic Citation
Molecules and Cells, vol. 41, no. 11, pp. 979-992
Publication Year
2018
Abstract
Potato (Solanum tuberosum L.) is the third most important food crop, and breeding drought-tolerant varieties is vital research goal. However, detailed molecular mechanisms in response to drought stress in potatoes are not well known. In this study, we developed EMS-mutagenized potatoes that showed significant tolerance to drought stress compared to the wild-type (WT) 'Desiree' cultivar. In addition, changes to transcripts as a result of drought stress in WT and drought-tolerant (DR) plants were investigated by de novo assembly using the Illumina platform. One-week-old WT and DR plants were treated with -1.8 Mpa polyethylene glycol-8000, and total RNA was prepared from plants harvested at 0, 6, 12, 24, and 48 h for subsequent RNA sequencing. In total, 61,100 transcripts and 5,118 differentially expressed genes (DEGs) displaying up- or down-regulation were identified in pairwise comparisons of WT and DR plants following drought conditions. Transcriptome profiling showed the number of DEGs with up-regulation and down-regulation at 909, 977, 1181, 1225 and 826 between WT and DR plants at 0, 6, 12, 24, and 48 h, respectively. Results of KEGG enrichment showed that the drought tolerance mechanism of the DR plant can mainly be explained by two aspects, the 'photosynthetic-antenna protein' and 'protein processing of the endoplasmic reticulum'. We also divided eight expression patterns in four pairwise comparisons of DR plants (DR0 vs DR6, DR12, DR24, DR48) under PEG treatment. Our comprehensive transcriptome data will further enhance our understanding of the mechanisms regulating drought tolerance in tetraploid potato cultivars.
Keyword
RNA-Seqdifferentially expressed genesdrought stressethyl-methanesulfonate-induced mutationpotato breeding
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.14348/molcells.2018.0312
Type
Article
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1. Journal Articles > Journal Articles
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