Identification of novel immunogenic proteins against Streptococcus parauberis in a zebrafish model by reverse vaccinology

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Title
Identification of novel immunogenic proteins against Streptococcus parauberis in a zebrafish model by reverse vaccinology
Author(s)
Young Su Kim; N K Yoon; N Karisa; S H Seo; Jeong Soo Lee; S S Yoo; I J Yoon; Y C Kim; Hong-Weon LeeJungoh Ahn
Bibliographic Citation
Microbial Pathogenesis, vol. 127, pp. 56-59
Publication Year
2019
Abstract
Streptococcus parauberis is the major infectious agent of streptococcosis in the olive flounder (Paralichthys olivaceus), causing serious economic damage. In this study, we identified potential vaccine candidates against S. parauberis by reverse vaccinology. In total, the 2 out of 21 proteins were identified as vaccine candidates from two available S. parauberis genomes. The membrane-anchored protein SEC10/PgrA and the metal ABC transporter substrate-binding lipoprotein mtsA were potent antigenic proteins based on western blotting with mouse-derived antiserum against whole bacteria of S. parauberis serotypes I and II. In particular, metal ABC transporter substrate-binding lipoprotein (mtsA) showed similar protective immunity to that of whole-cell bacterins against S. parauberis in a zebrafish model. These results suggest that mtsA may be considered as a novel candidate in the development of vaccines against S. parauberis.
Keyword
Metal ABC transporter substrate-binding lipoproteinReverse vaccinologyStreptococcus parauberisSubunit vaccineZebrafish
ISSN
0882-4010
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.micpath.2018.11.053
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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