Development of novel on-line capillary gas chromatography-based analysis method for volatile organic compounds produced by aerobic fermentation

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dc.contributor.authorHyeok Won Lee-
dc.contributor.authorJung Ho Park-
dc.contributor.authorHee-Suk Lee-
dc.contributor.authorChun Sug Kim-
dc.contributor.authorJin Gyeom Lee-
dc.contributor.authorWon-Kyo Kim-
dc.contributor.authorKyoung Hwa Ryu-
dc.contributor.authorJungoh Ahn-
dc.contributor.authorEun Gyo Lee-
dc.contributor.authorS W Kim-
dc.contributor.authorJ M Jeon-
dc.contributor.authorY H Yang-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorHong-Weon Lee-
dc.date.accessioned2019-01-23T16:31:08Z-
dc.date.available2019-01-23T16:31:08Z-
dc.date.issued2019-
dc.identifier.issn13891723-
dc.identifier.uri10.1016/j.jbiosc.2018.07.007ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/18283-
dc.description.abstractMany volatile compounds, such as isoprene, a precursor used in the synthesis of natural rubber, have been produced through fermentation using genetically engineered microorganisms. Despite this biotechnological success, measuring the concentrations of volatile compounds during fermentation is difficult because of their high volatility. In current systems, off-line analytical methods usually lead to product loss, whereas on-line methods raise the production cost due to the requirement of complex devices. Here, we developed a novel on-line gas chromatography (GC)-based system for analyzing the concentration of isoprene with the aim to minimize the cost and requirement for devices as compared to current strategies. In this system, a programmable logic controller is used to combine conventional GC with a syringe pump module (SPM) directly connected to the exhaust pipe of the fermentor, and isoprene-containing samples are continuously pumped from the SPM into the GC using an air cylinder recycle stream. We showed that this novel system enables isoprene analysis during fermentation with convenient equipment and without the requirement of an expensive desorption tube. Furthermore, this system may be extended to the detection of other volatile organic compounds in fermentation or chemical processes.-
dc.publisherSoc Bioscience Bioengineering Japan-
dc.titleDevelopment of novel on-line capillary gas chromatography-based analysis method for volatile organic compounds produced by aerobic fermentation-
dc.title.alternativeDevelopment of novel on-line capillary gas chromatography-based analysis method for volatile organic compounds produced by aerobic fermentation-
dc.typeArticle-
dc.citation.titleJournal of Bioscience and Bioengineering-
dc.citation.number1-
dc.citation.endPage127-
dc.citation.startPage121-
dc.citation.volume127-
dc.contributor.affiliatedAuthorHyeok Won Lee-
dc.contributor.affiliatedAuthorJung Ho Park-
dc.contributor.affiliatedAuthorJin Gyeom Lee-
dc.contributor.affiliatedAuthorWon-Kyo Kim-
dc.contributor.affiliatedAuthorKyoung Hwa Ryu-
dc.contributor.affiliatedAuthorJungoh Ahn-
dc.contributor.affiliatedAuthorEun Gyo Lee-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.affiliatedAuthorHong-Weon Lee-
dc.contributor.alternativeName이혁원-
dc.contributor.alternativeName박정호-
dc.contributor.alternativeName이희석-
dc.contributor.alternativeName김천석-
dc.contributor.alternativeName이진겸-
dc.contributor.alternativeName김원교-
dc.contributor.alternativeName류경화-
dc.contributor.alternativeName안정오-
dc.contributor.alternativeName이은교-
dc.contributor.alternativeName김선원-
dc.contributor.alternativeName전종민-
dc.contributor.alternativeName양영훈-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName이홍원-
dc.identifier.bibliographicCitationJournal of Bioscience and Bioengineering, vol. 127, no. 1, pp. 121-127-
dc.identifier.doi10.1016/j.jbiosc.2018.07.007-
dc.subject.keywordIsoprene-
dc.subject.keywordIsoprene production-
dc.subject.keywordOn-line monitoring-
dc.subject.keywordProgrammable logic controller-
dc.subject.keywordSyringe pump module-
dc.subject.localIsoprene-
dc.subject.localIsoprene production-
dc.subject.localOn-line monitoring-
dc.subject.localProgrammable logic controller-
dc.subject.localSyringe pump module-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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