Production of lipid containing high levels of docosahexaenoic acid by cultivation of Aurantiochytrium sp. KRS101 using Jerusalem artichoke extract

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Title
Production of lipid containing high levels of docosahexaenoic acid by cultivation of Aurantiochytrium sp. KRS101 using Jerusalem artichoke extract
Author(s)
Jung Hyun Ju; Baek Rock Oh; Seung-Kyu Ryu; Sun-Yeon Heo; Su-Yeon Kim; W K Hong; Chul Ho KimJeong-Woo Seo
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 23, no. 6, pp. 726-732
Publication Year
2018
Abstract
In the present study, we evaluated extract of Jerusalem artichoke tubers (JAT) as a substrate for the production of lipid containing high levels of docosahexaenoic acid (DHA) by cultivated Aurantiochytrium sp. KRS101, an oleaginous protist. The optimal conditions for cultivation determined using response surface methods were as follows: pH, 5.9; enzyme loading, 282.6; and temperature, 27.7°C. The maximal levels of lipid (16.4 g/L) and productivity (3.6 g/L/d) were obtained during fed-batch fermentation, which achieved a DHA yield of 7.9 g/L (>48% of total fatty acids by weight). Moreover, JAT was more economical, reducing costs of expensive yeast extract as a nitrogen source by about 40%. These results suggest that JAT may be further explored as a low-cost feedstock for lipid production using microalgae strains.
Keyword
docosahexaenoic acidJerusalem artichoke tuberslipidoleaginous Aurantiochytrium
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12257-018-0419-x
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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