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- Title
- Production of lipid containing high levels of docosahexaenoic acid by cultivation of Aurantiochytrium sp. KRS101 using Jerusalem artichoke extract
- Author(s)
- Jung Hyun Ju; Baek Rock Oh; Seung-Kyu Ryu; Sun-Yeon Heo; Su-Yeon Kim; W K Hong; Chul Ho Kim; Jeong-Woo Seo
- Bibliographic Citation
- Biotechnology and Bioprocess Engineering, vol. 23, no. 6, pp. 726-732
- Publication Year
- 2018
- Abstract
- In the present study, we evaluated extract of Jerusalem artichoke tubers (JAT) as a substrate for the production of lipid containing high levels of docosahexaenoic acid (DHA) by cultivated Aurantiochytrium sp. KRS101, an oleaginous protist. The optimal conditions for cultivation determined using response surface methods were as follows: pH, 5.9; enzyme loading, 282.6; and temperature, 27.7°C. The maximal levels of lipid (16.4 g/L) and productivity (3.6 g/L/d) were obtained during fed-batch fermentation, which achieved a DHA yield of 7.9 g/L (>48% of total fatty acids by weight). Moreover, JAT was more economical, reducing costs of expensive yeast extract as a nitrogen source by about 40%. These results suggest that JAT may be further explored as a low-cost feedstock for lipid production using microalgae strains.
- Keyword
- docosahexaenoic acidJerusalem artichoke tuberslipidoleaginous Aurantiochytrium
- ISSN
- 1226-8372
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/s12257-018-0419-x
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
- Files in This Item:
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