DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yae Jin Yoon | - |
dc.contributor.author | Young-Min Han | - |
dc.contributor.author | Jiyeon Choi | - |
dc.contributor.author | Yu Jin Lee | - |
dc.contributor.author | Jieun Yun | - |
dc.contributor.author | Su-Kyung Lee | - |
dc.contributor.author | Chang Woo Lee | - |
dc.contributor.author | Jong Soon Kang | - |
dc.contributor.author | Seung-Wook Chi | - |
dc.contributor.author | Jeong Hee Moon | - |
dc.contributor.author | Sangku Lee | - |
dc.contributor.author | Dong Cho Han | - |
dc.contributor.author | Byoung-Mog Kwon | - |
dc.date.accessioned | 2019-04-09T16:30:13Z | - |
dc.date.available | 2019-04-09T16:30:13Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 0006-2952 | - |
dc.identifier.uri | 10.1016/j.bcp.2019.01.017 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/18433 | - |
dc.description.abstract | Metastasis is the leading cause of cancer mortality and cancer cell migration is an essential stage of metastasis. We identified benproperine (Benp, a clinically used antitussive drug) as an inhibitor of cancer cell migration and an anti-metastatic agent. Benp selectively inhibited cancer cell migration and invasion, which also suppressed metastasis of cancer cells in animal models. Actin-related protein 2/3 complex subunit 2 (ARPC2) was identified as a molecular target of Benp by affinity column chromatography with Benp-tagged Sepharose beads. Benp bound directly to ARPC2 in cells, which was validated by pull-down assay using Benp-biotin and label-free biochemical methods such as the drug affinity responsive target stability (DARTS) and cellular thermal shift assay (CETSA). Benp inhibited Arp2/3 function, showing disruption of lamellipodial structure and inhibition of actin polymerization. Unlike Arp2/3 inhibitors, Benp selectively inhibited the migration of cancer cells but not normal cells. ARPC2-knockdown cancer cells showed defective cell migration and suppressed metastasis in an animal model. Therefore, ARPC2 is a potential target for anti-metastatic therapy, and Benp has the clinical potential to block metastasis. Furthermore, Benp is a useful agent for studying the functions of the Arp2/3 complex in cancer cell migration and metastasis. | - |
dc.publisher | Elsevier | - |
dc.title | Benproperine, an ARPC2 inhibitor, suppresses cancer cell migration and tumor metastasis = ARPC2 저해제인 벤프로페린의 암 전이 효과 | - |
dc.title.alternative | Benproperine, an ARPC2 inhibitor, suppresses cancer cell migration and tumor metastasis | - |
dc.type | Article | - |
dc.citation.title | Biochemical Pharmacology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 59 | - |
dc.citation.startPage | 46 | - |
dc.citation.volume | 163 | - |
dc.contributor.affiliatedAuthor | Yae Jin Yoon | - |
dc.contributor.affiliatedAuthor | Young-Min Han | - |
dc.contributor.affiliatedAuthor | Jiyeon Choi | - |
dc.contributor.affiliatedAuthor | Yu Jin Lee | - |
dc.contributor.affiliatedAuthor | Su-Kyung Lee | - |
dc.contributor.affiliatedAuthor | Chang Woo Lee | - |
dc.contributor.affiliatedAuthor | Jong Soon Kang | - |
dc.contributor.affiliatedAuthor | Seung-Wook Chi | - |
dc.contributor.affiliatedAuthor | Jeong Hee Moon | - |
dc.contributor.affiliatedAuthor | Sangku Lee | - |
dc.contributor.affiliatedAuthor | Dong Cho Han | - |
dc.contributor.affiliatedAuthor | Byoung-Mog Kwon | - |
dc.contributor.alternativeName | 윤예진 | - |
dc.contributor.alternativeName | 한영민 | - |
dc.contributor.alternativeName | 최지연 | - |
dc.contributor.alternativeName | 이유진 | - |
dc.contributor.alternativeName | 윤지은 | - |
dc.contributor.alternativeName | 이수경 | - |
dc.contributor.alternativeName | 이창우 | - |
dc.contributor.alternativeName | 강종순 | - |
dc.contributor.alternativeName | 지승욱 | - |
dc.contributor.alternativeName | 문정희 | - |
dc.contributor.alternativeName | 이상구 | - |
dc.contributor.alternativeName | 한동초 | - |
dc.contributor.alternativeName | 권병목 | - |
dc.identifier.bibliographicCitation | Biochemical Pharmacology, vol. 163, no. 1, pp. 46-59 | - |
dc.identifier.doi | 10.1016/j.bcp.2019.01.017 | - |
dc.subject.keyword | ARPC2 | - |
dc.subject.keyword | Arp2/3 complex | - |
dc.subject.keyword | Benproperine | - |
dc.subject.keyword | Drug repurposing | - |
dc.subject.keyword | Metastasis | - |
dc.subject.local | ARPC2 | - |
dc.subject.local | Arp2/3 complex | - |
dc.subject.local | Benproperine | - |
dc.subject.local | Drug Repurposing | - |
dc.subject.local | drug repurposing | - |
dc.subject.local | Drug repurposing | - |
dc.subject.local | metastasis | - |
dc.subject.local | Metastasis | - |
dc.description.journalClass | Y | - |
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