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- Title
- Rapid tool for identification of bacterial strains using Fourier transform infrared spectroscopy on genomic DNA
- Author(s)
- Jiyoung Lee; Myung-Suk Ahn; Y L Lee; Eun Yee Jie; Song-Gun Kim; Suk Weon Kim
- Bibliographic Citation
- Journal of Applied Microbiology, vol. 126, no. 3, pp. 864-871
- Publication Year
- 2019
- Abstract
- AIMS: We developed a new rapid and reliable method for identifying bacteria using a combination of Fourier transform infrared (FT-IR) spectroscopy of bacterial genomic DNA and multivariate analysis.
METHODS AND RESULTS: FT-IR spectra of genomic DNA from four type strains of Pseudomonas spp., three type strains of Escherichia spp. and two type strains of Bacillus spp. were analysed in the 4000-400 cm-1 region. Spectral differences were found in the frequency regions of N-H stretching (amide I), C=O stretching vibrations (amide II) and PO2 - ionized asymmetric and symmetric stretching. Partial least squares discriminant analysis of the FT-IR spectra showed that the microbial strains could be discriminated by hierarchical clustering analysis.
CONCLUSIONS: FT-IR spectral analysis of bacterial genomic DNA has potential for the rapid identification of bacteria at the genus and species levels.
SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports a new bacterial identification method using multivariate analysis of FT-IR spectra of bacterial genomic DNA.
- Keyword
- Fourier transform infrared spectroscopybacterial identificationgenomic DNAhierarchical clustering analysismultivariate analysis
- ISSN
- 1364-5072
- Publisher
- Wiley
- DOI
- http://dx.doi.org/10.1111/jam.14171
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
- Files in This Item:
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