Rapid tool for identification of bacterial strains using Fourier transform infrared spectroscopy on genomic DNA

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Title
Rapid tool for identification of bacterial strains using Fourier transform infrared spectroscopy on genomic DNA
Author(s)
Jiyoung Lee; Myung-Suk Ahn; Y L Lee; Eun Yee JieSong-Gun KimSuk Weon Kim
Bibliographic Citation
Journal of Applied Microbiology, vol. 126, no. 3, pp. 864-871
Publication Year
2019
Abstract
AIMS: We developed a new rapid and reliable method for identifying bacteria using a combination of Fourier transform infrared (FT-IR) spectroscopy of bacterial genomic DNA and multivariate analysis. METHODS AND RESULTS: FT-IR spectra of genomic DNA from four type strains of Pseudomonas spp., three type strains of Escherichia spp. and two type strains of Bacillus spp. were analysed in the 4000-400 cm-1 region. Spectral differences were found in the frequency regions of N-H stretching (amide I), C=O stretching vibrations (amide II) and PO2 - ionized asymmetric and symmetric stretching. Partial least squares discriminant analysis of the FT-IR spectra showed that the microbial strains could be discriminated by hierarchical clustering analysis. CONCLUSIONS: FT-IR spectral analysis of bacterial genomic DNA has potential for the rapid identification of bacteria at the genus and species levels. SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports a new bacterial identification method using multivariate analysis of FT-IR spectra of bacterial genomic DNA.
Keyword
Fourier transform infrared spectroscopybacterial identificationgenomic DNAhierarchical clustering analysismultivariate analysis
ISSN
1364-5072
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/jam.14171
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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