Identification of MazF homologue in Legionella pneumophila which cleaves RNA at the AACU sequence

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dc.contributor.authorM Shaku-
dc.contributor.authorJung Ho Park-
dc.contributor.authorM Inouye-
dc.contributor.authorY Yamaguchi-
dc.date.accessioned2019-04-09T16:30:32Z-
dc.date.available2019-04-09T16:30:32Z-
dc.date.issued2018-
dc.identifier.issn14641801-
dc.identifier.uri10.1159/000497146ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/18504-
dc.description.abstractMazF is a sequence-specific endoribonuclease that is widely conserved in bacteria and archaea. Here, we found an MazF homologue (MazF-lp; LPO-p0114) in Legionella pneumophila. The mazF-lp gene overlaps 14 base pairs with the upstream gene mazE-lp (MazE-lp; LPO-p0115). The induction of mazF-lp caused cell growth arrest, while mazE-lp co-induction recovered cell growth in Escherichia coli. In vivo and in vitro primer extension experiments showed that MazF-lp is a sequence-specific endoribonuclease cleaving RNA at AACU. The endoribonuclease activity of purified MazF-lp was inhibited by purified MazE-lp. We found that MazE-lp and the MazEF-lp complex specifically bind to the palindromic sequence present in the 5'-untranslated region of the mazEF-lp operon. MazE-lp and MazEF-lp both likely function as a repressor for the mazEF-lp operon and for other genes, including icmR, whose gene product functions as a secretion chaperone for the IcmQ pore-forming protein, by specifically binding to the palindromic sequence in 5'-UTR of these genes.-
dc.publisherKarger-
dc.titleIdentification of MazF homologue in Legionella pneumophila which cleaves RNA at the AACU sequence-
dc.title.alternativeIdentification of MazF homologue in Legionella pneumophila which cleaves RNA at the AACU sequence-
dc.typeArticle-
dc.citation.titleJournal of Molecular Microbiology and Biotechnology-
dc.citation.number6-
dc.citation.endPage280-
dc.citation.startPage269-
dc.citation.volume28-
dc.contributor.affiliatedAuthorJung Ho Park-
dc.contributor.alternativeNameShaku-
dc.contributor.alternativeName박정호-
dc.contributor.alternativeNameInouye-
dc.contributor.alternativeNameYamaguchi-
dc.identifier.bibliographicCitationJournal of Molecular Microbiology and Biotechnology, vol. 28, no. 6, pp. 269-280-
dc.identifier.doi10.1159/000497146-
dc.subject.keywordMazF-
dc.subject.keywordToxin-
dc.subject.keywordToxin-antitoxin system-
dc.subject.localMazF-
dc.subject.localToxin-
dc.subject.localToxin-antitoxin system-
dc.description.journalClassY-
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Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
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