Naked-eye detection of pandemic influenza a (pH1N1) virus by polydiacetylene (PDA)-based paper sensor as a point-of-care diagnostic platform

Abstract

We have developed polydiacetylene (PDA)-based colorimetric biosensor for point-of-care testing (POCT) to detect high infectious pH1N1 virus among influenza A virus with the naked eye by color change. For this purpose, we first prepared PDA-PVDF membrane by immobilizing PDA onto the PVDF membrane through photo-polymerization, and then conjugated an antibody specifically binding to the influenza A virus into this membrane to form PDA-paper chips. This PDA-paper chips exhibited unique chromatic properties involving a color change from blue to red under various external conditions (temperature and pHs). Especially, we showed the color change of this paper chip in the presence of pH1N1 virus, confirming its potential as a POCT device. Furthermore, we have developed POCT systems based on PDA-paper chips by developing program, which is possible to visually detect viruses using only PDA-paper chips at a high concentration and detect and analyze them at low concentrations using both paper chips and program (App.). Further, this program will be updated to enable quantitative analysis of the virus.