Arazyme suppresses hepatic steatosis and steatohepatitis in diet-induced non-alcoholic fatty liver disease-like mouse model = 비알콜성 지방간 모델 마우스에서 아라자임의 지방간 및 지방간염 억제 효능

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Title
Arazyme suppresses hepatic steatosis and steatohepatitis in diet-induced non-alcoholic fatty liver disease-like mouse model = 비알콜성 지방간 모델 마우스에서 아라자임의 지방간 및 지방간염 억제 효능
Author(s)
Hua Li; Wonbeak Yoo; Hye-Mi Park; Soo Youn Lim; D H Shin; Seokho Kim; Ho Yong ParkTae Sook Jeong
Bibliographic Citation
International Journal of Molecular Sciences, vol. 20, pp. 2325-2325
Publication Year
2019
Abstract
Arazyme, a metalloprotease from the spider Nephila clavata, exerts hepatoprotective activity in CCL4-induced acute hepatic injury. This study investigated the hepatoprotective e ects in high-fat diet (HFD)-induced non-alcoholic fatty liver disease-like C57BL/6J mice. The mice were randomly divided into four groups (n = 10/group): the normal diet group, the HFD group, the arazyme group (HFD with 0.025% arazyme), and the milk thistle (MT) group (HFD with 0.1% MT). Dietary supplementation of arazyme for 13 weeks significantly lowered plasma triglyceride (TG) and non-esterified fatty acid levels. Suppression of HFD-induced hepatic steatosis in the arazyme group was caused by the reduced hepatic TG and total cholesterol (TC) contents. Arazyme supplementation decreased hepatic lipogenesis-related gene expression, sterol regulatory element-binding transcription protein 1 (Srebf1), fatty acid synthase (Fas), acetyl-CoA carboxylase 1 (Acc1), stearoyl-CoA desaturase-1 (Scd1), Scd2, glycerol-3-phosphate acyltransferase (Gpam), diacylglycerol O-acyltransferase 1 (Dgat1), and Dgat2. Arazyme directly reduced palmitic acid (PA)-induced TG accumulation in HepG2 cells. Arazyme suppressed macrophage infiltration and tumor necrosis factor (Tnfa), interleukin-1 (Il1b), and chemokine-ligand-2 (Ccl2) expression in the liver, and inhibited secretion of TNF and expression of inflammatory mediators, Tnfa, Il1b, Ccl2, Ccl3, Ccl4, and Ccl5, in PA-induced RAW264.7 cells. Arazyme e ectively protected hepatic steatosis and steatohepatitis by inhibiting SREBP-1-mediated lipid accumulation and macrophage-mediated inflammation.
Keyword
arazymediet therapynon-alcoholic fatty liver diseaseSREBP-1steatohepatitissteatosis
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms20092325
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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