TSPYL5-mediated inhibition of p53 promotes human endothelial cell function

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Title
TSPYL5-mediated inhibition of p53 promotes human endothelial cell function
Author(s)
Hee Jun Na; Chung Eun Yeum; Han-Seop KimJungwoon Lee; Jae Yun Kim; Yee Sook Cho
Bibliographic Citation
Angiogenesis, vol. 22, no. 2, pp. 281-293
Publication Year
2019
Abstract
Testis-specific protein, Y-encoded like (TSPYL) family proteins (TSPYL1-6), which are members of the nucleosome assembly protein superfamily, have been determined to be involved in the regulation of various cellular functions. However, the potential role of TSPYL family proteins in endothelial cells (ECs) has not been determined. Here, we demonstrated that the expression of TSPYL5 is highly enriched in human ECs such as human umbilical vein endothelial cells (HUVECs) and human pluripotent stem cell-differentiated ECs (hPSC-ECs). Importantly, TSPYL5 overexpression was shown to promote EC proliferation and functions, such as migration and tube formation, by downregulating p53 expression. Adriamycin-induced senescence was markedly blocked by TSPYL5 overexpression. In addition, the TSPYL5 depletion-mediated loss of EC functions was blocked by p53 inhibition. Significantly, TSPYL5 overexpression promoted angiogenesis in Matrigel plug and wound repair in a mouse skin wound healing model in vivo. Our results suggest that TSPYL5, a novel angiogenic regulator, plays a key role in maintaining endothelial integrity and function. These findings extend the understanding of TSPYL5-dependent mechanisms underlying the regulation of p53-related functions in ECs.
Keyword
TSPYL5Endothelial cellsProliferationAngiogenesisp53
ISSN
0969-6970
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10456-018-9656-z
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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