Intestinibaculum porci gen. nov., sp. nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine

Abstract

A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102 T , was isolated from the small intestine of a swine. Optimal growth occurred at 37°C and pH 7.0. Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%. The comparative analysis of 16S rRNA gene sequences showed that strain SG0102 T was most closely related to Kandleria vitulina DSM 20405 T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053 T (91.1%), Sharpea azabuensis KCTC 15217 T (91.0%), and Eggerthia catenaformis DSM 5348 T (89.6%). The average nucleotide identity values between strain SG0102 T and related species, K. vitulina DSM 20405 T , C. mitsuokai KCTC 5053 T , S. azabuensis KCTC 15217 T , and E. catenaformis DSM 5348 T , were 71.0, 69.3, 70.0, and 69.2%, respectively. The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102 T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia. The DNA G + C content of the strain SG0102 T was 39.5 mol%. The major cellular fatty acids (> 10%) of strain SG0102 T were C 16:0 , C 16:0 dimethyl acetal, and C 18:2 ω 9/12c. The cell wall peptidoglycan of strain SG0102 T contained the meso-diaminopimelic acid. The strain SG0102 T produced lactic acid as a major end product of fermentation. These distinct phenotypic and phylogenetic properties suggest that strain SG0102 T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp. nov. is proposed. The type strain is SG0102 T (= KCTC 15725 T = NBRC 113396 T ).