Anti-osteoporotic effects of kukoamine B isolated from Lycii Radicis cortex extract on osteoblast and osteoclast cells and ovariectomized osteoporosis model mice

Cited 12 time in scopus
Metadata Downloads
Title
Anti-osteoporotic effects of kukoamine B isolated from Lycii Radicis cortex extract on osteoblast and osteoclast cells and ovariectomized osteoporosis model mice
Author(s)
E Park; J Kim; M C Kim; S Yeo; J Kim; S Park; M Jo; C W Choi; H S Jin; Sang Woo Lee; W Y Li; J W Lee; J H Park; D Huh; S Y Jeong
Bibliographic Citation
International Journal of Molecular Sciences, vol. 20, no. 11, pp. 2784-2784
Publication Year
2019
Abstract
Osteoporosis is an abnormal bone remodeling condition characterized by decreased bone density, which leads to high risks of fracture. Previous study has demonstrated that Lycii Radicis Cortex (LRC) extract inhibits bone loss in ovariectomized (OVX) mice by enhancing osteoblast differentiation. A bioactive compound, kukoamine B (KB), was identified from fractionation of an LRC extract as a candidate component responsible for an anti-osteoporotic effect. This study investigated the anti-osteoporotic effects of KB using in vitro and in vivo osteoporosis models. KB treatment significantly increased the osteoblastic differentiation and mineralized nodule formation of osteoblastic MC3T3-E1 cells, while it significantly decreased the osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. The effects of KB on osteoblastic and osteoclastic differentiations under more physiological conditions were also examined. In the co-culture of MC3T3-E1 cells and monocytes, KB promoted osteoblast differentiation but did not affect osteoclast differentiation. In vivo experiments revealed that KB significantly inhibited OVX-induced bone mineral density loss and restored the impaired bone structural properties in osteoporosis model mice. These results suggest that KB may be a potential therapeutic candidate for the treatment of osteoporosis.
Keyword
osteoporosisherbal medicinekukoamine Bosteoblastosteoclastbone mineral densityovariectomized mice
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms20112784
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > International Biological Material Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.