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- Spastin contributes to neural development through the regulation of microtubule dynamics in the primary cilia of neural stem cells
- Bohyeon Jeong; Tae Hwan Kim; Dae Soo Kim; W H Shin; Jae-Ran Lee; Nam-Soon Kim; Da Yong Lee
- Bibliographic Citation
- Neuroscience, vol. 411, pp. 76-85
- Publication Year
- Spastin is a microtubule-severing enzyme encoded by SPAST, which is broadly expressed in various cell
types originated from multiple organs. Even though SPAST is well known as a regulator of the axon growth and arborization
in neurons and a genetic factor of hereditary spastic paraplegia, it also takes part in a wide range of other cellular
functions including the regulation of cell division and proliferation. In this study, we investigated a novel
biological role of spastin in developing brain using Spast deficient mouse embryonic neural stem cells (NSCs) and
perinatal mouse brain. We found that the expression of spastin begins at early embryonic stages in mouse brain. Using
Spast shRNA treated NSCs and mouse brain, we showed that Spast deficiency leads to decrease of NSC proliferation
and neuronal lineage differentiation. Finally, we found that spastin controls NSC proliferation by regulating microtubule
dynamics in primary cilia. Collectively, these data demonstrate that spastin controls brain development by the
regulation of NSC functions at early developmental stages.
- spastin; neural stem cell; brain development; primary cilia; microtubule.
- Appears in Collections:
- Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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