Biocatalytic synthesis of non-natural monoterpene O-glycosides exhibiting superior antibacterial and antinematodal properties = 우수한 할균활성 및 살충활성을 나타내는 non-natural monoterpene O-glycosides의 biocatalytic synthesis

Abstract

A promiscuous Bacillus glycosyltransferase (YjiC) was explored for the enzymatic synthesis of monoterpene O-glycosides in vitro and in vivo. YjiC converted seven monoterpenes into 41 different sugar-conjugated novel glycoside derivatives. The wholecell biotransformation of the same set of monoterpenes exhibited robust enzyme activity to synthesize O-glucosyl derivatives from Escherichia coli. These newly synthesized selected monoterpene-Oglucosyl derivatives exhibited enhanced antibacterial activities against human pathogenic bacteria and antinematodal activities against pine wood nematode Bursaphelenchus xylophilus.