PAS1-modified optical SIS sensor for highly sensitive and specific detection of toluene

Cited 2 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorT T Dung-
dc.contributor.authorUi Jin Lee-
dc.contributor.authorMyung Hee Kim-
dc.contributor.authorK E Kim-
dc.contributor.authorH M Cho-
dc.contributor.authorC D Fermin-
dc.contributor.authorD H Kim-
dc.contributor.authorMoonil Kim-
dc.date.accessioned2019-10-28T16:30:04Z-
dc.date.available2019-10-28T16:30:04Z-
dc.date.issued2019-
dc.identifier.issn09565663-
dc.identifier.uri10.1016/j.bios.2019.111469ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/18859-
dc.description.abstractWe report on a novel solution immersed silicon (SIS) sensor modified with bio-receptor to detect toluene. To perform this approach, bio-receptor PAS1 which specifically interacts with toluene was chosen as a capture agent for SIS ellipsometric sensing. We constructed wild PAS1 and mutant PAS1 (F46A and F79Y) which are toluene binding-defective. Especially, we utilized an easily accessible capturing approach based on silica binding peptide (SBP) for direct immobilization of PAS1 on the SiO2 surfaces. After the immobilization of SBP-tagged PAS1 to the sensing layers, PAS1-based SIS sensor was evaluated for its ability to recognize toluene. As a result, a significant up-shift in Psi (Ψ) was clearly observed with a low limit of detection (LOD) of 0.1?μM, when treated with toluene on wild PAS1-surface, but not on mutant PAS1-sensing layers, indicating the selective interactions between PAS1 and toluene molecule. The PAS1-SIS sensor showed no changes in Psi (Ψ), if any, negligible, when exposed to benzene, phenol, xylene and 4-nitrophenol as negative controls, thereby demonstrating the specificity of interaction between PAS1 and toluene. Taken together, our results strongly indicate that PAS1-modified ellipsometry sensor can provide a high fidelity system for the accurate and selective detection of toluene.-
dc.publisherElsevier-
dc.titlePAS1-modified optical SIS sensor for highly sensitive and specific detection of toluene-
dc.title.alternativePAS1-modified optical SIS sensor for highly sensitive and specific detection of toluene-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number0-
dc.citation.endPage111469-
dc.citation.startPage111469-
dc.citation.volume141-
dc.contributor.affiliatedAuthorUi Jin Lee-
dc.contributor.affiliatedAuthorMyung Hee Kim-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.alternativeNameDung-
dc.contributor.alternativeName이의진-
dc.contributor.alternativeName김명희-
dc.contributor.alternativeName김균언-
dc.contributor.alternativeName조현모-
dc.contributor.alternativeNameFermin-
dc.contributor.alternativeName김동형-
dc.contributor.alternativeName김문일-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 141, pp. 111469-111469-
dc.identifier.doi10.1016/j.bios.2019.111469-
dc.subject.keywordBio-receptor-
dc.subject.keywordEllipsometry-
dc.subject.keywordPAS1-
dc.subject.keywordSolution immersed silicon-
dc.subject.keywordToluene-
dc.subject.localBio-receptor-
dc.subject.localEllipsometry-
dc.subject.localPAS1-
dc.subject.localSolution immersed silicon-
dc.subject.localToluene-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.