Microbial production of medium chain fructooligosaccharides by recombinant yeast secreting bacterial inulosucrase

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Title
Microbial production of medium chain fructooligosaccharides by recombinant yeast secreting bacterial inulosucrase
Author(s)
Hyunjun Ko; Jung Hoon BaeBong Hyun SungMi Jin Kim; Hyun-Ju Park; Jung Hoon Sohn
Bibliographic Citation
Enzyme and Microbial Technology, vol. 130, pp. 109364-109364
Publication Year
2019
Abstract
A high yielding and straightforward production system of fructooligosaccharide (FOS) was developed for industrial production of prebiotics. To increase conversion yield of FOS from sucrose, recombinant yeast secreting inulosucrase from Lactobacillus reuteri (LrInu) were constructed. Efficient secretion of LrInu was achieved by truncation of both amino- and carboxy-termini (LrInuΔNC) and by introducing an optimal secretion signal. The recombinant yeast produced 220 U/mL of recombinant LrInuΔNC into culture medium during fed-batch fermentation. By direct fermentation of recombinant yeast in medium containing sucrose, 128.4g/L of FOS was produced with 85.6% conversion yield from 300g/L sucrose, and the highest titer was 152.6g/L from 400g/L sucrose. The degree of polymerization of generated FOS was 2-20 indicating medium chain (mcFOS) range. This is the first report of industrially applicable production of mcFOS by recombinant yeast secreting bacterial inulosucrase.
Keyword
Direct fermentationFructooligosaccharidesInulosucraseMedium chainSaccharomyces cerevisiae
ISSN
0141-0229
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.enzmictec.2019.109364
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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