DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung Hyun Ju | - |
dc.contributor.author | Baek Rock Oh | - |
dc.contributor.author | Dong-Jin Ko | - |
dc.contributor.author | Sun-Yeon Heo | - |
dc.contributor.author | Jong-Jea Lee | - |
dc.contributor.author | Y M Kim | - |
dc.contributor.author | K Yang | - |
dc.contributor.author | Jeong-Woo Seo | - |
dc.contributor.author | W K Hong | - |
dc.contributor.author | Chul Ho Kim | - |
dc.date.accessioned | 2019-10-28T16:30:12Z | - |
dc.date.available | 2019-10-28T16:30:12Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 2211-9264 | - |
dc.identifier.uri | 10.1016/j.algal.2019.101474 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/18887 | - |
dc.description.abstract | We isolated and characterized an oleaginous Thraustochytrid microalga. Based on 18s rRNA sequencing, we classified this strain as Schizochytrium sp. SH103. Growth of these cells in a 5L fermentor with a microbubble-type sparger (MTS) efficiently increased the oxygen transfer coefficient (OTC), while inducing only low external stress. For fermentation under conditions of low agitation (100 rpm) and aeration (3L/min), the OTC was 8.6-fold higher using a MTS than a general type sparger (GTS). Under these same conditions, dry cell weight (DCW) productivity (0.54 vs. 1.26g/L·h) and total lipid productivity (0.29 vs. 0.61g/L·h) were each about 2.2-fold higher using a MTS than a GTS. Under conditions that optimized the OTC in a MTS fermentor (876 h -1), DCW productivity, total lipid productivity and DHA productivity were each 1.3-fold greater than non-optimized condition. Fed-batch fermentation experiments indicated the maximal DCW was 100.6g/L at 48 h, maximal DCW productivity was 2.1g/L·h, maximal lipid content was 48.6g/L at 48 h, and lipid productivity was 1.0 g/L·h. Additionally, the results of experiments using a 50L MTS fermentor (96.0g/L DCW and 44.3g/L total lipids at 48h) were similar to those using the 5L fermentor. Our results suggest that use of a MTS may help to achieve the efficient fermentation of heterotrophic microalgae for high cell densities. | - |
dc.publisher | Elsevier | - |
dc.title | Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger | - |
dc.title.alternative | Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger | - |
dc.type | Article | - |
dc.citation.title | Algal Research-Biomass Biofuels and Bioproducts | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 101474 | - |
dc.citation.startPage | 101474 | - |
dc.citation.volume | 41 | - |
dc.contributor.affiliatedAuthor | Jung Hyun Ju | - |
dc.contributor.affiliatedAuthor | Baek Rock Oh | - |
dc.contributor.affiliatedAuthor | Dong-Jin Ko | - |
dc.contributor.affiliatedAuthor | Sun-Yeon Heo | - |
dc.contributor.affiliatedAuthor | Jong-Jea Lee | - |
dc.contributor.affiliatedAuthor | Jeong-Woo Seo | - |
dc.contributor.affiliatedAuthor | Chul Ho Kim | - |
dc.contributor.alternativeName | 주정현 | - |
dc.contributor.alternativeName | 오백록 | - |
dc.contributor.alternativeName | 고동진 | - |
dc.contributor.alternativeName | 허선연 | - |
dc.contributor.alternativeName | 이종재 | - |
dc.contributor.alternativeName | 김영민 | - |
dc.contributor.alternativeName | 양권석 | - |
dc.contributor.alternativeName | 서정우 | - |
dc.contributor.alternativeName | 홍원경 | - |
dc.contributor.alternativeName | 김철호 | - |
dc.identifier.bibliographicCitation | Algal Research-Biomass Biofuels and Bioproducts, vol. 41, pp. 101474-101474 | - |
dc.identifier.doi | 10.1016/j.algal.2019.101474 | - |
dc.subject.keyword | Schizochytrium sp. | - |
dc.subject.keyword | Bio-oil | - |
dc.subject.keyword | Microbubble | - |
dc.subject.keyword | Oxygen transfer coefficient | - |
dc.subject.local | Schizochytrium sp. | - |
dc.subject.local | Bio-oil | - |
dc.subject.local | bio-oil | - |
dc.subject.local | Microbubble | - |
dc.subject.local | microbubble | - |
dc.subject.local | Oxygen transfer coefficient | - |
dc.description.journalClass | Y | - |
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