Real-time PCR quantification of spliced X-box binding protein 1 (XBP1) using a universal primer method

Cited 18 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSeung-Bin Yoon-
dc.contributor.authorYoung-Ho Park-
dc.contributor.authorSeon A Choi-
dc.contributor.authorPil Soo Jeong-
dc.contributor.authorJae Jin Cha-
dc.contributor.authorSanghoon Lee-
dc.contributor.authorSeung Hwan Lee-
dc.contributor.authorJong Hee Lee-
dc.contributor.authorBo Woong Sim-
dc.contributor.authorBon Sang Koo-
dc.contributor.authorSang Je Park-
dc.contributor.authorYoung Jeon Lee-
dc.contributor.authorYoung-Hyun Kim-
dc.contributor.authorJung Joo Hong-
dc.contributor.authorJi-Su Kim-
dc.contributor.authorYeung Bae Jin-
dc.contributor.authorJae Won Huh-
dc.contributor.authorSang-Rae Lee-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorSun-Uk Kim-
dc.date.accessioned2019-10-28T16:30:16Z-
dc.date.available2019-10-28T16:30:16Z-
dc.date.issued2019-
dc.identifier.issn19326203-
dc.identifier.uri10.1371/journal.pone.0219978ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/18902-
dc.description.abstractX-box binding protein 1 (XBP1) mRNA processing plays a crucial role in the unfolded protein response (UPR), which is activated in response to endoplasmic reticulum (ER) stress. Upon accumulation of the UPR-converted XBP1 mRNA splicing from an unspliced (u) XBP1 (inactive) isoform to the spliced (s) XBP1 (active) isoform, inositol-requiring enzyme 1 α (IRE1α) removes a 26-nucleotide intron from uXBP1 mRNA. Recent studies have reported the assessment of ER stress by examining the ratio of sXBP1 to uXBP1 mRNA (s/uXBP1 ratio) via densitometric analysis of PCR bands relative to increased levels of sXBP1 to uXBP1 using a housekeeping gene for normalization. However, this measurement is visualized by gel electrophoresis, making it very difficult to quantify differences between the two XBP1 bands and complicating data interpretation. Moreover, most commonly used housekeeping genes display an unacceptably high variable expression pattern of the s/uXBP1 ratio under different experimental conditions, such as various phases of development and different cell types, limiting their use as internal controls. For a more quantitative determination of XBP1 splicing activity, we measured the expression levels of total XBP1 (tXBP1: common region of s/uXBP1) and sXBP1 via real-time PCR using specific primer sets. We also designed universal real-time PCR primer sets capable of amplifying a portion of each u/s/tXBP1 mRNA that is highly conserved in eukaryotes, including humans, monkeys, cows, pigs, and mice. Therefore, we provide a more convenient and easily approachable quantitative real-time PCR method that can be used in various research fields to assess ER stress.-
dc.publisherPublic Library of Science-
dc.titleReal-time PCR quantification of spliced X-box binding protein 1 (XBP1) using a universal primer method-
dc.title.alternativeReal-time PCR quantification of spliced X-box binding protein 1 (XBP1) using a universal primer method-
dc.typeArticle-
dc.citation.titlePLoS One-
dc.citation.number7-
dc.citation.endPagee0219978-
dc.citation.startPagee0219978-
dc.citation.volume14-
dc.contributor.affiliatedAuthorSeung-Bin Yoon-
dc.contributor.affiliatedAuthorYoung-Ho Park-
dc.contributor.affiliatedAuthorSeon A Choi-
dc.contributor.affiliatedAuthorPil Soo Jeong-
dc.contributor.affiliatedAuthorJae Jin Cha-
dc.contributor.affiliatedAuthorSanghoon Lee-
dc.contributor.affiliatedAuthorSeung Hwan Lee-
dc.contributor.affiliatedAuthorJong Hee Lee-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.affiliatedAuthorBon Sang Koo-
dc.contributor.affiliatedAuthorSang Je Park-
dc.contributor.affiliatedAuthorYoung Jeon Lee-
dc.contributor.affiliatedAuthorYoung-Hyun Kim-
dc.contributor.affiliatedAuthorJung Joo Hong-
dc.contributor.affiliatedAuthorJi-Su Kim-
dc.contributor.affiliatedAuthorYeung Bae Jin-
dc.contributor.affiliatedAuthorJae Won Huh-
dc.contributor.affiliatedAuthorSang-Rae Lee-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.alternativeName윤승빈-
dc.contributor.alternativeName박영호-
dc.contributor.alternativeName최선아-
dc.contributor.alternativeName정필수-
dc.contributor.alternativeName차재진-
dc.contributor.alternativeName이상훈-
dc.contributor.alternativeName이승환-
dc.contributor.alternativeName이종희-
dc.contributor.alternativeName심보웅-
dc.contributor.alternativeName구본상-
dc.contributor.alternativeName박상제-
dc.contributor.alternativeName이영전-
dc.contributor.alternativeName김영현-
dc.contributor.alternativeName홍정주-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName진영배-
dc.contributor.alternativeName허재원-
dc.contributor.alternativeName이상래-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName김선욱-
dc.identifier.bibliographicCitationPLoS One, vol. 14, no. 7, pp. e0219978-e0219978-
dc.identifier.doi10.1371/journal.pone.0219978-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.