Studies on antioxidant activity and in vitro inhibitory activity of tyrosinase and collagenase in Artocarpus nitidus subsp. lingnaensis (Merr.) F.M. Jarrett using 4 parameter logistic = 변수 분석을 통한 아토카푸스 니티두스 추출물과 분획물의 항산화, 타이로시나제 및 콜라제나제 In Vitro 저해활성 연구

Abstract

In this study, the antioxidative and inhibitory activity of tyrosinase and collagenase for the solvent extract and silica column fractions of Artocarpus nitidus were evaluated. The activities were quantified using the 4 parameter logistic. LC/MS analysis showed that the major component of the fractions was polyphenol and the total polyphenol content of the extract was 48.1 ± 2.6 mg GAE/g. The radical scavenging activities (SC50) for 1,1-diphenyl-2-picrylhydrazyl of the extract, fraction-1 and fraction-2 were 16.7, 42.0 and 10.1 μg/mL, respectively. The value for fraction-2 was the closest to ascorbic acid (1.5 μg/mL). The tyrosinase inhibitory activity of the extracts and the fractions showed IC50 of 64.9, 0.9 and 1.2 μg/mL, respectively, and overall activity was higher than that of kojic acid (7.4 μg/mL) and arbutin (119.0 μg/mL). In the experiment by zebrafish embryo, the whitening activity of fraction-2 (27.5%) was higher than that of kojic acid (18.6%), and there was no adverse effect up to 500 μg/mL of fraction-2. For the collagenase inhibitory activity, the samples showed IC50 of 139.8, 20.6, and 16.8 μg/mL, respectively, which were competitive to 1, 10-Phenanthroline (55.4 μg/mL). The extract and fraction-2 showed IC50 of 61.8 and 67.1 μg/mL for elastase. These results suggest that A. nitidus extract can be used as a cosmetic material useful for antioxidant, whitening, and prevention of skin aging without adverse effects.