Sequence variation analysis of flowering-time genes in two radish lines with different flowering time = 개화시기가 상이한 두 개의 무 계통간 개화유전자 서열변이 분석

Abstract

It is important for radish to have late flowering characteristics especially in the case of spring and winter cultivars. To understand late flowering characteristics of radish at the molecular level the flowering time genes of two radish lines (NH-JS1 and NH-JS2) with different flowering time were compared by re-sequencing their genomes. There were a total of 872,587 SNPs and 194,637 INDELs between the two lines. The SNP density of each chromosome was relatively uniform throughout, but the region with low SNP density was found at the end of R3 and the middle of R9. To compare the flowering time genes of the two lines, we first looked for the flowering time genes in radish using Arabidopsis thaliana flowering time genes. As a result, homologs of radish were found for most flowering time genes, but FRIGIDA was not found. Among 224 radish flowering time gene-homologs found, 97 genes showed more than one sequence difference (SNP or INDEL) between the two lines, and 127 genes had no difference. In particular, no sequence differences were found in FT, CO, and FLC, core flowering time control genes. Rs350520 (FVE), Rs193800 (CURLY LEAF) and Rs255320 (ATX1) with more than 100 sequence variations were expected to have a significant effect on flowering time difference between the two lines. These results will be of great help in understanding the flowering timing difference between the two lines at the molecular level.