Increased CD68/TGFβ co-expressing microglia/macrophages after transient middle cerebral artery occlusion in rhesus monkeys = 붉은털 원숭이에서 일시적인 중간 대뇌 동맥 폐색 후 CD68과 TGFβ를 동시 발현하면 미세아교세포/대식세포가 증가

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Title
Increased CD68/TGFβ co-expressing microglia/macrophages after transient middle cerebral artery occlusion in rhesus monkeys = 붉은털 원숭이에서 일시적인 중간 대뇌 동맥 폐색 후 CD68과 TGFβ를 동시 발현하면 미세아교세포/대식세포가 증가
Author(s)
Hyeon-Gu Yeo; Jung Joo HongYoungjeon Lee; K S Yi; Chang Yeop Jeon; Junghyung Park; Jinyoung Won; Jincheol Seo; Yu-Jin Ahn; Keonwoo Kim; Seung Ho Baek; Eun Ha Hwang; Green Kim; Yeung Bae Jin; Kang Jin JeongBon Sang KooPhilyong KangKyung Seob LimSun-Uk KimJae Won HuhYoung-Hyun Kim; Yeonghoon Son; Ji-Su Kim; C H Choi; S H Cha; Sang-Rae Lee
Bibliographic Citation
Experimental Neurobiology, vol. 28, no. 4, pp. 458-473
Publication Year
2019
Abstract
The function of microglia/macrophages after ischemic stroke is poorly understood. This study examines the role of microglia/macrophages in the focal infarct area after transient middle cerebral artery occlusion (MCAO) in rhesus monkeys. We measured infarct volume and neurological function by magnetic resonance imaging (MRI) and non-human primate stroke scale (NHPSS), respectively, to assess temporal changes following MCAO. Activated phagocytic microglia/macrophages were examined by immunohistochemistry in post-mortem brains (n=6 MCAO, n=2 controls) at 3 and 24 hours (acute stage), 2 and 4 weeks (subacute stage), and 4, and 20 months (chronic stage) following MCAO. We found that the infarct volume progressively decreased between 1 and 4 weeks following MCAO, in parallel with the neurological recovery. Greater presence of cluster of differentiation 68 (CD68)-expressing microglia/macrophages was detected in the infarct lesion in the subacute and chronic stage, compared to the acute stage. Surprisingly, 98~99% of transforming growth factor beta (TGFβ) was found co-localized with CD68-expressing cells. CD68-expressing microglia/macrophages, rather than CD206+ cells, may exert anti-inflammatory effects by secreting TGFβ after the subacute stage of ischemic stroke. CD68+ microglia/macrophages can therefore be used as a potential therapeutic target.
Keyword
InflammationMacaca mulattaMicrogliaStrokeTransforming growth factor beta
ISSN
1226-2560
Publisher
South Korea
DOI
http://dx.doi.org/10.5607/en.2019.28.4.458
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
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