Cytoprotective effects of an aqueous extracts from atrina pectinate meat in H2O2-induced cxidative stress in a human hepatocyte

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Title
Cytoprotective effects of an aqueous extracts from atrina pectinate meat in H2O2-induced cxidative stress in a human hepatocyte
Author(s)
E J Shin; E J Han; H J Han; Kyungsook Jung; S J Heo; E A Kim; K N Kim; S C Kim; M J Seo; I S Kwak; M J Kim; G Ahn; W W Lee
Bibliographic Citation
Advances in Experimental Medicine and Biology, vol. 1155, pp. 661-674
Publication Year
2019
Abstract
In the present study, we investigated the antioxidant activity of an aqueous extract from Atrina pectinate meat (APW) against H2O2-induced oxidative stress in a human hepatocyte. The extraction yield of APW was 30.01 ± 0.83% and which contained the highest taurine content among free amino acid contents. APW led to the high antioxidant activity showing 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, good reducing power and oxygen radical absorbance capacity (ORAC) value. Also, the results showed that APW improved the cell viability decreased by H2O2 stimulation as well as the reduction of intracellular reactive oxygen species (ROS) generation in hepatocytes. Additionally, APW up-regulated the production of antioxidant mechanisms related enzymes such as catalase and superoxide dismutase (SOD), compared to the only H2O2-treated hepatocytes. Moreover, APW increased the expressions of nuclear Nrf2 and cytosolic HO-1 in H2O2-treated hepatocytes. Interestingly, the treatment of ZnPP, a HO-1 inhibitor abolished the cell viability and intracellular ROS generation induced by APW treatment. In conclusion, this study suggests that APW protects H2O2 induced oxidative stress via up-regulating of Nrf2/HO-1 signal pathway in hepatocytes.
Keyword
Anti-oxidantAtrina pectinateHepatocytesNrf2/HO-1 signal pathway
ISSN
0065-2598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/978-981-13-8023-5_58
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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