Evaluation of feasibility of using the bacteriophage T4 lysozyme to improve the hydrolysis and biochemical methane potential of secondary sludge = 2차 슬러지의 가수분해와 메탄 생산 향상을 위한 박테리오파지 유래 세포벽 분해효소의 실효성 평가

Cited 2 time in scopus
Metadata Downloads
Title
Evaluation of feasibility of using the bacteriophage T4 lysozyme to improve the hydrolysis and biochemical methane potential of secondary sludge = 2차 슬러지의 가수분해와 메탄 생산 향상을 위한 박테리오파지 유래 세포벽 분해효소의 실효성 평가
Author(s)
S Kim; Seung Gyun Woo; J Lee; Dae-Hee Lee; S Hwang
Bibliographic Citation
Energies, vol. 12, pp. 3644-3644
Publication Year
2019
Abstract
Anaerobic digestion (AD) of secondary sludge is a rate-limiting step due to the bacterial cell wall. In this study, experiments were performed to characterize secondary sludges from three wastewater treatment plants (WWTPs), and to investigate the feasibility of using bacteriophage lysozymes to speed up AD by accelerating the degradation of bacterial cell walls. Protein was the main organic material (67.7% of volatile solids in the sludge). The bacteriophage T4 lysozyme (T4L) was tested for hydrolysis and biochemical methane potential. Variations in the volatile suspended solid (VSS) concentration and biogas production were monitored. The VSS reduction efficiencies by hydrolysis using T4L for 72 h increased and ranged from 17.8% to 26.4%. Biogas production using T4L treated sludges increased and biogas production was increased by as much as 82.4%. Biogas production rate also increased, and the average reaction rate coefficient of first-order kinetics was 0.56 ± 0.02/d, which was up to 47.5% higher compared to the untreated samples at the maximum. Alphaproteobacteria, Betaproteobacteria, Flavobacteriia, Gammaproteobacteria, and Sphingobacteriia were major microbial classes in all sludges. The interpretation of the microbial community structure indicated that T4L treatment is likely to increase the rate of cell wall digestion.
Keyword
sludge hydrolysisbiochemical methane potential (BMP)bacterial community
ISSN
1996-1073
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/en12193644
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.