Differential regulation of TLE3 in sertoli cells of the testes during postnatal development

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Differential regulation of TLE3 in sertoli cells of the testes during postnatal development
S Lee; Hoon Jang; S Moon; O H Lee; S LeeJ Lee; C Park; D W Seol; H Song; K Hong; J H Kim; S J Uhm; D R Lee; Jeong Woong Lee; Y Choi
Bibliographic Citation
Cells, vol. 8, no. 10, pp. 1156-1156
Publication Year
Spermatogenesis is a process by which haploid cells differentiate from germ cells in the seminiferous tubules of the testes. TLE3, a transcriptional co-regulator that interacts with DNA-binding factors, plays a role in the development of somatic cells. However, no studies have shown its role during germ cell development in the testes. Here, we examined TLE3 expression in the testes during spermatogenesis. TLE3 was highly expressed in mouse testes and was dynamically regulated in different cell types of the seminiferous tubules, spermatogonia, spermatids, and Sertoli cells, but not in the spermatocytes. Interestingly, TLE3 was not detected in Sertoli cells on postnatal day 7 (P7) but was expressed from P10 onward. The microarray analysis showed that the expression of numerous genes changed upon TLE3 knockdown in a Sertoli cell line TM4. These include 1597 up-regulated genes and 1452 down-regulated genes in TLE3-knockdown TM4 cells. Ingenuity Pathway Analysis (IPA) showed that three factors were up-regulated and two genes were down-regulated upon TLE3 knockdown in TM4 cells. The abnormal expression of the three factors is associated with cellular malfunctions such as abnormal differentiation and Sertoli cell formation. Thus, TLE3 is differentially expressed in Sertoli cells and plays a crucial role in regulating cell-specific genes involved in the differentiation and formation of Sertoli cells during testicular development.
Sertoli cellTLE3Testisspermatogenesis
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Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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