A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs

Cited 18 time in scopus
Metadata Downloads
Title
A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs
Author(s)
I C Cho; H B Park; Jin Seop Ahn; S H Han; J B Lee; H T Lim; C K Yoo; E J Jung; Dong-Hwan Kim; W S Sun; Y Ramayo-Caldas; S G Kim; Y J Kang; Y K Kim; H S Shin; P N Seong; I S Hwang; P Y Park; S Hwang; S S Lee; Y C Ryu; J H Lee; M S Ko; K Lee; G Andersson; M Perez-Enciso; Jeong Woong Lee
Bibliographic Citation
PLoS Genetics, vol. 15, no. 10, pp. 1008279-1008279
Publication Year
2019
Abstract
Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5'-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.
ISSN
1553-7390
Publisher
Public Library of Science
DOI
http://dx.doi.org/10.1371/journal.pgen.1008279
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.