Production of bio-based isoprene by the mevalonate pathway cassette in Ralstonia eutropha

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Title
Production of bio-based isoprene by the mevalonate pathway cassette in Ralstonia eutropha
Author(s)
Hyeok Won LeeJung Ho Park; Hee Seok Lee; Wonho Choi; Sung-Hwa Seo; I D Anggraini; Eui Sung Choi; Hong-Weon Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 29, no. 10, pp. 1656-1664
Publication Year
2019
Abstract
Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. Ralstonia eutropha can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of R. eutropha H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter (1.9 ± 0.24 μg/l) than when using the lac promoter (1.5 ± 0.2 μg/l). Additionally, the use of three J5 promoters was more efficient (3.8 ± 0.18 μg/l) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that R. eutropha can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.
Keyword
MVA pathwayRalstonia eutrophafermentationisoprene productionpromoter
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1909.09002
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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