A human pathogenic bacterium Shigella proliferates in plants through adoption of type III effectors for shigellosis

Cited 8 time in scopus
Metadata Downloads
Title
A human pathogenic bacterium Shigella proliferates in plants through adoption of type III effectors for shigellosis
Author(s)
Sung Hee Jo; Jiyoung Lee; E Park; D W Kim; Dae-Hee LeeChoong-Min Ryu; D Choi; Jeong Mee Park
Bibliographic Citation
Plant Cell and Environment, vol. 42, no. 11, pp. 2962-2978
Publication Year
2019
Abstract
Shigella, which infects primates, can be transmitted via fresh vegetables; however, its molecular interactions with plants have not been elucidated. Here, we show that four Shigella strains, Shigella boydii, Shigella sonnei, Shigella flexneri 2a, and S. flexneri 5a, proliferate at different levels in Arabidopsis thaliana. Microscopic studies revealed that these bacteria were present inside leaves and damaged plant cells. Green fluorescent protein (GFP)-tagged S. boydii and S. flexneri 5a colonized leaves only, whereas S. flexneri 2a colonized both leaves and roots. Using Shigella mutants lacking type III secretion systems (T3SSs), we found that T3SSs that regulate the pathogenesis of shigellosis in humans also play a central role in bacterial proliferation in Arabidopsis. Strikingly, the immunosuppressive activity of two T3S effectors, OspF and OspG, was required for proliferation of Shigella in Arabidopsis. Of note, delivery of OspF or OspG effectors inside plant cells upon Shigella inoculation was confirmed using a split GFP system. These findings demonstrate that the human pathogen Shigella can proliferate in plants by adapting immunosuppressive machinery used in the original host human.
Keyword
PAMP-triggered immunityShigella spp.T3S effectorsalternative hostenteropathogenic bacteriaproliferationsplit GFPtrans-kingdom pathogenesistype III secretion system (T3SS)Arabidopsis plants
ISSN
0140-7791
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/pce.13603
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.