Chamaejasmine isolated from Wikstroemia dolichantha diels suppresses 2,4-dinitrofluoro-benzene-induced atopic dermatitis in SKH-1 hairless mice
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- Title
- Chamaejasmine isolated from Wikstroemia dolichantha diels suppresses 2,4-dinitrofluoro-benzene-induced atopic dermatitis in SKH-1 hairless mice
- Author(s)
- T Y Kim; N J Park; J Jegal; Choi Sangho; Sang Woo Lee; J Hang; S N Kim; M H Yang
- Bibliographic Citation
- Biomolecules, vol. 9, no. 11, pp. 697-697
- Publication Year
- 2019
- Abstract
- Plants of the genus Wikstroemia have long been used as traditional medicines to treat diseases like pneumonia, rheumatism, and bronchitis. This study was designed to determine the effect of chamaejasmine, a biflavonoid present in W. dolichantha, on atopic dermatitis (AD)-like skin lesions in a 2,4-dinitrochlorobenzene (DNCB)-induced murine model of AD. Initially, we examined the anti-allergic activities of ten flavonoids from W. dolichantha by measuring β-hexosaminidase release from RBL-2H3 cells. Subsequently, an SKH-1 hairless mouse model of AD was developed based on the topical application of DNCB. Chamaejasmine (0.5%) or pimecrolimus (1%, positive control) were applied to dorsal skins of DNCB-sensitized AD mice for two weeks. Serum IL-4 and IgE levels were determined using enzyme-linked immunosorbent assay kits and transepidermal water loss (TEWL) and skin hydration were measured using a Tewameter TM210 and a SKIN-O-MAT, respectively. Of the ten flavonoids isolated from W. dolichantha, chamaejasmine most potently inhibited DNP-specific IgE-induced degranulation in RBL-2H3 cells. Topical administration of chamaejasmine attenuated the clinical symptoms of DNCB-induced dermatitis (i.e., itching, dryness, erythema, and edema). Histological analyses demonstrated that dermal thickness and mast cell infiltration in dermis were significantly reduced by chamaejasmine. In addition, 0.5% chamaejasmine inhibited DNCB-induced increases in total IL-4 and IgE levels in serum, improved skin barrier function, and increased epidermis moisture. Our findings suggest chamaejasmine might be an effective therapeutic agent for the treatment of atopic diseases.
- Keyword
- 2,4-dinitrochlorobenzeneAtopic dermatitisChamaejasmineInterleukin 4Skin barrier functionWikstroemia dolichantha
- ISSN
- 2218-273X
- Publisher
- MDPI
- DOI
- http://dx.doi.org/10.3390/biom9110697
- Type
- Article
- Appears in Collections:
- Ochang Branch Institute > Division of National Bio-Infrastructure > International Biological Material Research Center > 1. Journal Articles
- Files in This Item:
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