Recent advances in the CRISPR genome editing tool set

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dc.contributor.authorSu Bin Moon-
dc.contributor.authorDo Yon Kim-
dc.contributor.authorJeong Heon Ko-
dc.contributor.authorYong Sam Kim-
dc.description.abstractGenome editing took a dramatic turn with the development of the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) system. The CRISPR-Cas system is functionally divided into classes 1 and 2 according to the composition of the effector genes. Class 2 consists of a single effector nuclease, and routine practice of genome editing has been achieved by the development of the Class 2 CRISPR-Cas system, which includes the type II, V, and VI CRISPR-Cas systems. Types II and V can be used for DNA editing, while type VI is employed for RNA editing. CRISPR techniques induce both qualitative and quantitative alterations in gene expression via the double-stranded breakage (DSB) repair pathway, base editing, transposase-dependent DNA integration, and gene regulation using the CRISPR-dCas or type VI CRISPR system. Despite significant technical improvements, technical challenges should be further addressed, including insufficient indel and HDR efficiency, off-target activity, the large size of Cas, PAM restrictions, and immune responses. If sophisticatedly refined, CRISPR technology will harness the process of DNA rewriting, which has potential applications in therapeutics, diagnostics, and biotechnology.-
dc.publisherSpringer-Nature Pub Group-
dc.titleRecent advances in the CRISPR genome editing tool set-
dc.title.alternativeRecent advances in the CRISPR genome editing tool set-
dc.citation.titleExperimental and Molecular Medicine-
dc.contributor.affiliatedAuthorSu Bin Moon-
dc.contributor.affiliatedAuthorDo Yon Kim-
dc.contributor.affiliatedAuthorJeong Heon Ko-
dc.contributor.affiliatedAuthorYong Sam Kim-
dc.identifier.bibliographicCitationExperimental and Molecular Medicine, vol. 51, no. 11, pp. 130-130-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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