Demethylation and derepression of genomic retroelements in the skeletal muscles of aged mice

Cited 11 time in scopus
Metadata Downloads
Title
Demethylation and derepression of genomic retroelements in the skeletal muscles of aged mice
Author(s)
Byungkuk Min; Kyuheum Jeon; Jung Sun ParkYong-Kook Kang
Bibliographic Citation
Aging Cell, vol. 18, no. 6, pp. e13042-e13042
Publication Year
2019
Abstract
Changes in DNA methylation influence the aging process and contribute to aging phenotypes, but few studies have been conducted on DNA methylation changes in conjunction with skeletal muscle aging. We explored the DNA methylation changes in a variety of retroelement families throughout aging (at 2, 20, and 28 months of age) in murine skeletal muscles by methyl-binding domain sequencing (MBD-seq). The two following contrasting patterns were observed among the members of each repeat family in superaged mice: (a) hypermethylation in weakly methylated retroelement copies and (b) hypomethylation in copies with relatively stronger methylation levels, representing a pattern of "regression toward the mean" within a single retroelement family. Interestingly, these patterns depended on the sizes of the copies. While the majority of the elements showed a slight increase in methylation, the larger copies (>5 kb) displayed evident demethylation. All these changes were not observed in T cells. RNA sequencing revealed a global derepression of retroelements during the late phase of aging (between 20 and 28 months of age), which temporally coincided with retroelement demethylation. Following this methylation drift trend of "regression toward the mean," aging tended to progressively lose the preexisting methylation differences and local patterns in the genomic regions that had been elaborately established during the early period of development.
Keyword
DNA methylationLINE1MBD-seqagingendogenous retrovirus (ERV)retroelementskeletal muscle
ISSN
1474-9718
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/acel.13042
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.