Thermomonas aquatica sp. nov., isolated from an industrial wastewater treatment plant

Abstract

A white-coloured, Gram-stain-negative, aerobic, rod-shaped bacterium (designated strain SY21T) was isolated from waste-activated sludge. Optimal growth occurred at 28°C and pH 7.0. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain SY21T exhibited 16S rRNA gene sequence similarities of 95.5-98.0% to Thermomonas species and clustered with the type species of the genus Thermomonas. In strain SY21T, the predominant respiratory quinone was ubiquinone Q-8, and the cellular fatty acids consisted mainly of iso-C15:0, C16:0, iso-C11:0 3-OH, summed feature 3 and summed feature 9. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was determined to be 67.9 mol% and the DNA-DNA relatedness between strain SY21T and the closest phylogenetically related strain, Thermomonas carbonis KCTC 42013T, was 35.0±0.1%. Based on the distinct phenotypic, chemotaxonomic and phylogenetic properties, strain SY21T represents a novel species of the genus Thermomonas, for which the name Thermomonas aquatica sp. nov. is proposed. The type strain is SY21T (=KCTC 62191T=NBRC 113114T).