Quantification of Enterocytozoon hepatopenaei (EHP) in penaeid shrimps from southeast Asia and Latin America using TaqMan probe-based quantitative PCR

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Title
Quantification of Enterocytozoon hepatopenaei (EHP) in penaeid shrimps from southeast Asia and Latin America using TaqMan probe-based quantitative PCR
Author(s)
P Piamsomboon; S K Choi; B Hanggono; Y L Nuraini; F Wati; K F J Tang; S Park; D Kwak; M H Rhee; J E Han; Ji Hyung Kim
Bibliographic Citation
Pathogens, vol. 8, no. 4, pp. 233-233
Publication Year
2019
Abstract
We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106-108 copies mg-1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101-103 copies mg-1 or mL-1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms
Keyword
Enterocytozoon hepatopenaeimicrosporidianqPCRshrimp parasite
ISSN
2076-0817
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/pathogens8040233
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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