Open Access@KRIBBDivision of Research on National ChallengesInfectious Disease Research Center1. Journal Articles
Quantification of Enterocytozoon hepatopenaei (EHP) in penaeid shrimps from southeast Asia and Latin America using TaqMan probe-based quantitative PCR
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- Title
- Quantification of Enterocytozoon hepatopenaei (EHP) in penaeid shrimps from southeast Asia and Latin America using TaqMan probe-based quantitative PCR
- Author(s)
- P Piamsomboon; S K Choi; B Hanggono; Y L Nuraini; F Wati; K F J Tang; S Park; D Kwak; M H Rhee; J E Han; Ji Hyung Kim
- Bibliographic Citation
- Pathogens, vol. 8, no. 4, pp. 233-233
- Publication Year
- 2019
- Abstract
- We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106-108 copies mg-1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101-103 copies mg-1 or mL-1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms
- ISSN
- 2076-0817
- Publisher
- MDPI
- Full Text Link
- http://dx.doi.org/10.3390/pathogens8040233
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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