Comprehensive profiling of surface gangliosides extracted from various cell lines by LC-MS/MS

Cited 10 time in scopus
Metadata Downloads
Title
Comprehensive profiling of surface gangliosides extracted from various cell lines by LC-MS/MS
Author(s)
J Lee; H Hwang; S Kim; J Hwang; J Yoon; D Yin; S I Choi; Y H Kim; Yong Sam Kim; H J An
Bibliographic Citation
Cells, vol. 8, no. 11, pp. 1323-1323
Publication Year
2019
Abstract
Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis. Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools. Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski. We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS. Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively. The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively. Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties. In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1). These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types.
Keyword
LC-MS/MScancer cellcell surfacegangliosideglycosylation
ISSN
2073-4409
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/cells8111323
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.