DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung Jin Yoon | - |
dc.contributor.author | D H Jo | - |
dc.contributor.author | Seung-Ho Park | - |
dc.contributor.author | Jun-Young Park | - |
dc.contributor.author | Yoo Kyung Lee | - |
dc.contributor.author | Moo-Seung Lee | - |
dc.contributor.author | Jeong Ki Min | - |
dc.contributor.author | Haiyoung Jung | - |
dc.contributor.author | Tae-Don Kim | - |
dc.contributor.author | Suk Ran Yoon | - |
dc.contributor.author | S W Chung | - |
dc.contributor.author | J H Kim | - |
dc.contributor.author | In Pyo Choi | - |
dc.contributor.author | Young-Jun Park | - |
dc.date.accessioned | 2020-02-07T16:30:39Z | - |
dc.date.available | 2020-02-07T16:30:39Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | 10.3389/fimmu.2019.02636 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/19173 | - |
dc.description.abstract | In host defense, it is crucial to maintain the acidity of the macrophage phagosome for effective bacterial clearance. However, the mechanisms governing phagosomal acidification upon exposure to gram-negative bacteria have not been fully elucidated. In this study, we demonstrate that in macrophages exposed to Escherichia coli, the thioredoxin-interacting protein (TXNIP)-associated inflammasome plays a role in pH modulation through the activated caspase-1-mediated inhibition of NADPH oxidase. While there was no difference in early-phase bacterial engulfment between Txnip knockout (KO) macrophages and wild-type (WT) macrophages, Txnip KO macrophages were less efficient at destroying intracellular bacteria in the late phase, and their phagosomes failed to undergo appropriate acidification. These phenomena were associated with reactive oxygen species production and were reversed by treatment with an NADPH oxidase inhibitor or a caspase inhibitor. In line with these results, Txnip KO mice were more susceptible to both intraperitoneally administered E. coli and sepsis induced by cecum ligation and puncture than WT mice. Taken together, this study suggests that the TXNIP-associated inflammasome-caspase-1 axis regulates NADPH oxidase to modulate the pH of the phagosome, controlling bacterial clearance by macrophages. | - |
dc.publisher | Frontiers Media Sa | - |
dc.title | Thioredoxin-interacting protein promotes phagosomal acidification upon exposure to Escherichia coli through inflammasome-mediated caspase-1 activation in macrophages | - |
dc.title.alternative | Thioredoxin-interacting protein promotes phagosomal acidification upon exposure to Escherichia coli through inflammasome-mediated caspase-1 activation in macrophages | - |
dc.type | Article | - |
dc.citation.title | Frontiers in Immunology | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 2636 | - |
dc.citation.startPage | 2636 | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Sung Jin Yoon | - |
dc.contributor.affiliatedAuthor | Seung-Ho Park | - |
dc.contributor.affiliatedAuthor | Jun-Young Park | - |
dc.contributor.affiliatedAuthor | Yoo Kyung Lee | - |
dc.contributor.affiliatedAuthor | Moo-Seung Lee | - |
dc.contributor.affiliatedAuthor | Jeong Ki Min | - |
dc.contributor.affiliatedAuthor | Haiyoung Jung | - |
dc.contributor.affiliatedAuthor | Tae-Don Kim | - |
dc.contributor.affiliatedAuthor | Suk Ran Yoon | - |
dc.contributor.affiliatedAuthor | In Pyo Choi | - |
dc.contributor.affiliatedAuthor | Young-Jun Park | - |
dc.contributor.alternativeName | 윤성진 | - |
dc.contributor.alternativeName | 조동현 | - |
dc.contributor.alternativeName | 박승호 | - |
dc.contributor.alternativeName | 박준영 | - |
dc.contributor.alternativeName | 이유경 | - |
dc.contributor.alternativeName | 이무승 | - |
dc.contributor.alternativeName | 민정기 | - |
dc.contributor.alternativeName | 정해용 | - |
dc.contributor.alternativeName | 김태돈 | - |
dc.contributor.alternativeName | 윤석란 | - |
dc.contributor.alternativeName | 정수월 | - |
dc.contributor.alternativeName | 김정훈 | - |
dc.contributor.alternativeName | 최인표 | - |
dc.contributor.alternativeName | 박영준 | - |
dc.identifier.bibliographicCitation | Frontiers in Immunology, vol. 10, pp. 2636-2636 | - |
dc.identifier.doi | 10.3389/fimmu.2019.02636 | - |
dc.subject.keyword | Escherichia coli | - |
dc.subject.keyword | caspase | - |
dc.subject.keyword | macrophage | - |
dc.subject.keyword | phagosome | - |
dc.subject.keyword | thioredoxin-interacting protein | - |
dc.subject.local | E. Coli | - |
dc.subject.local | E. coli | - |
dc.subject.local | E.coli | - |
dc.subject.local | Escherichia Coli | - |
dc.subject.local | Escherichia coli | - |
dc.subject.local | Escherichia coli. | - |
dc.subject.local | escherichia coil | - |
dc.subject.local | escherichia coli | - |
dc.subject.local | Caspase | - |
dc.subject.local | Caspases | - |
dc.subject.local | caspase | - |
dc.subject.local | macrophage | - |
dc.subject.local | macrophages | - |
dc.subject.local | Macrophage | - |
dc.subject.local | Macrophages | - |
dc.subject.local | phagosome | - |
dc.subject.local | Thioredoxin-interacting protein | - |
dc.subject.local | thioredoxin-interacting protein | - |
dc.subject.local | thioredoxin interacting protein | - |
dc.description.journalClass | Y | - |
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