DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pil Soo Jeong | - |
dc.contributor.author | Seung-Bin Yoon | - |
dc.contributor.author | Mun-Hyeong Lee | - |
dc.contributor.author | Hee Chang Son | - |
dc.contributor.author | Hwal Yong Lee | - |
dc.contributor.author | Sanghoon Lee | - |
dc.contributor.author | Bon Sang Koo | - |
dc.contributor.author | Kang Jin Jeong | - |
dc.contributor.author | Jong Hee Lee | - |
dc.contributor.author | Yeung Bae Jin | - |
dc.contributor.author | Bong-Seok Song | - |
dc.contributor.author | Ji-Su Kim | - |
dc.contributor.author | Sun-Uk Kim | - |
dc.contributor.author | D B Koo | - |
dc.contributor.author | Bo Woong Sim | - |
dc.date.accessioned | 2020-02-07T16:30:48Z | - |
dc.date.available | 2020-02-07T16:30:48Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 2167-8359 | - |
dc.identifier.uri | 10.7717/peerj.8143 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/19215 | - |
dc.description.abstract | Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated embryos significantly improved developmental competence, including blastocyst formation rate, blastomere number, ICM/TE ratio, and cellular survival rate, compared to non-aggregated embryos. Investigation into the relationship between embryo aggregation and stress conditions revealed that mitochondrial function increased, and oxidative and endoplasmic reticulum (ER)-stress decreased compared to 1X (non-aggregated embryos) blastocysts. In addition, 3X (three-embryo aggregated) blastocysts increased the expression of pluripotency, anti-apoptosis, and implantation related genes, and decreased expression of pro-apoptosis related genes. Therefore, these findings indicate that embryo aggregation regulates in vitro stress conditions to increase developmental competence and contributes to the in vitro production of high-quality embryos and the large-scale production of transgenic and chimeric pigs. | - |
dc.publisher | PeerJ Inc | - |
dc.title | Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs | - |
dc.title.alternative | Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs | - |
dc.type | Article | - |
dc.citation.title | PeerJ | - |
dc.citation.number | 0 | - |
dc.citation.endPage | e8143 | - |
dc.citation.startPage | e8143 | - |
dc.citation.volume | 7 | - |
dc.contributor.affiliatedAuthor | Pil Soo Jeong | - |
dc.contributor.affiliatedAuthor | Seung-Bin Yoon | - |
dc.contributor.affiliatedAuthor | Mun-Hyeong Lee | - |
dc.contributor.affiliatedAuthor | Hee Chang Son | - |
dc.contributor.affiliatedAuthor | Hwal Yong Lee | - |
dc.contributor.affiliatedAuthor | Sanghoon Lee | - |
dc.contributor.affiliatedAuthor | Bon Sang Koo | - |
dc.contributor.affiliatedAuthor | Kang Jin Jeong | - |
dc.contributor.affiliatedAuthor | Jong Hee Lee | - |
dc.contributor.affiliatedAuthor | Yeung Bae Jin | - |
dc.contributor.affiliatedAuthor | Bong-Seok Song | - |
dc.contributor.affiliatedAuthor | Ji-Su Kim | - |
dc.contributor.affiliatedAuthor | Sun-Uk Kim | - |
dc.contributor.affiliatedAuthor | Bo Woong Sim | - |
dc.contributor.alternativeName | 정필수 | - |
dc.contributor.alternativeName | 윤승빈 | - |
dc.contributor.alternativeName | 이문형 | - |
dc.contributor.alternativeName | 손희창 | - |
dc.contributor.alternativeName | 이활용 | - |
dc.contributor.alternativeName | 이상훈 | - |
dc.contributor.alternativeName | 구본상 | - |
dc.contributor.alternativeName | 정강진 | - |
dc.contributor.alternativeName | 이종희 | - |
dc.contributor.alternativeName | 진영배 | - |
dc.contributor.alternativeName | 송봉석 | - |
dc.contributor.alternativeName | 김지수 | - |
dc.contributor.alternativeName | 김선욱 | - |
dc.contributor.alternativeName | 구덕본 | - |
dc.contributor.alternativeName | 심보웅 | - |
dc.identifier.bibliographicCitation | PeerJ, vol. 7, pp. e8143-e8143 | - |
dc.identifier.doi | 10.7717/peerj.8143 | - |
dc.subject.keyword | Blastocyst quality | - |
dc.subject.keyword | Endoplasmic reticulum stress | - |
dc.subject.keyword | Mitochondrial function | - |
dc.subject.keyword | Pig embryo aggregation | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.subject.local | Blastocyst quality | - |
dc.subject.local | Endoplasmic reticulum (ER) stress | - |
dc.subject.local | endoplasmic reticulum stress | - |
dc.subject.local | endoplasmic reticulum-stress | - |
dc.subject.local | Endoplasmic reticulum stress | - |
dc.subject.local | Mitochondrial function | - |
dc.subject.local | Pig embryo aggregation | - |
dc.subject.local | Reactive oxidative species | - |
dc.subject.local | Reactive oxygen species(ROS) | - |
dc.subject.local | Reactive oxygen species | - |
dc.subject.local | Reactive Oxygen Species (ROS) | - |
dc.subject.local | Reactive Oxygen Species | - |
dc.subject.local | ROS | - |
dc.subject.local | Reactive oxygen species (ROS) | - |
dc.subject.local | reactive oxygen species | - |
dc.subject.local | reactive oxygen species (ROS) | - |
dc.description.journalClass | Y | - |
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