Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs

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dc.contributor.authorPil Soo Jeong-
dc.contributor.authorSeung-Bin Yoon-
dc.contributor.authorMun-Hyeong Lee-
dc.contributor.authorHee Chang Son-
dc.contributor.authorHwal Yong Lee-
dc.contributor.authorSanghoon Lee-
dc.contributor.authorBon Sang Koo-
dc.contributor.authorKang Jin Jeong-
dc.contributor.authorJong Hee Lee-
dc.contributor.authorYeung Bae Jin-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorJi-Su Kim-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorD B Koo-
dc.contributor.authorBo Woong Sim-
dc.date.accessioned2020-02-07T16:30:48Z-
dc.date.available2020-02-07T16:30:48Z-
dc.date.issued2019-
dc.identifier.issn2167-8359-
dc.identifier.uri10.7717/peerj.8143ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19215-
dc.description.abstractEmbryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated embryos significantly improved developmental competence, including blastocyst formation rate, blastomere number, ICM/TE ratio, and cellular survival rate, compared to non-aggregated embryos. Investigation into the relationship between embryo aggregation and stress conditions revealed that mitochondrial function increased, and oxidative and endoplasmic reticulum (ER)-stress decreased compared to 1X (non-aggregated embryos) blastocysts. In addition, 3X (three-embryo aggregated) blastocysts increased the expression of pluripotency, anti-apoptosis, and implantation related genes, and decreased expression of pro-apoptosis related genes. Therefore, these findings indicate that embryo aggregation regulates in vitro stress conditions to increase developmental competence and contributes to the in vitro production of high-quality embryos and the large-scale production of transgenic and chimeric pigs.-
dc.publisherPeerJ Inc-
dc.titleEmbryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs-
dc.title.alternativeEmbryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs-
dc.typeArticle-
dc.citation.titlePeerJ-
dc.citation.number0-
dc.citation.endPagee8143-
dc.citation.startPagee8143-
dc.citation.volume7-
dc.contributor.affiliatedAuthorPil Soo Jeong-
dc.contributor.affiliatedAuthorSeung-Bin Yoon-
dc.contributor.affiliatedAuthorMun-Hyeong Lee-
dc.contributor.affiliatedAuthorHee Chang Son-
dc.contributor.affiliatedAuthorHwal Yong Lee-
dc.contributor.affiliatedAuthorSanghoon Lee-
dc.contributor.affiliatedAuthorBon Sang Koo-
dc.contributor.affiliatedAuthorKang Jin Jeong-
dc.contributor.affiliatedAuthorJong Hee Lee-
dc.contributor.affiliatedAuthorYeung Bae Jin-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorJi-Su Kim-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.alternativeName정필수-
dc.contributor.alternativeName윤승빈-
dc.contributor.alternativeName이문형-
dc.contributor.alternativeName손희창-
dc.contributor.alternativeName이활용-
dc.contributor.alternativeName이상훈-
dc.contributor.alternativeName구본상-
dc.contributor.alternativeName정강진-
dc.contributor.alternativeName이종희-
dc.contributor.alternativeName진영배-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName구덕본-
dc.contributor.alternativeName심보웅-
dc.identifier.bibliographicCitationPeerJ, vol. 7, pp. e8143-e8143-
dc.identifier.doi10.7717/peerj.8143-
dc.subject.keywordBlastocyst quality-
dc.subject.keywordEndoplasmic reticulum stress-
dc.subject.keywordMitochondrial function-
dc.subject.keywordPig embryo aggregation-
dc.subject.keywordReactive oxygen species-
dc.subject.localBlastocyst quality-
dc.subject.localEndoplasmic reticulum (ER) stress-
dc.subject.localendoplasmic reticulum stress-
dc.subject.localendoplasmic reticulum-stress-
dc.subject.localEndoplasmic reticulum stress-
dc.subject.localMitochondrial function-
dc.subject.localPig embryo aggregation-
dc.subject.localReactive oxidative species-
dc.subject.localReactive oxygen species(ROS)-
dc.subject.localReactive oxygen species-
dc.subject.localReactive Oxygen Species (ROS)-
dc.subject.localReactive Oxygen Species-
dc.subject.localROS-
dc.subject.localReactive oxygen species (ROS)-
dc.subject.localreactive oxygen species-
dc.subject.localreactive oxygen species (ROS)-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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