Development of monoclonal antibody specific to foot-and-mouth disease virus type A for serodiagnosis

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Title
Development of monoclonal antibody specific to foot-and-mouth disease virus type A for serodiagnosis
Author(s)
Q T Nguyen; Jihyun Yang; J W Byun; H M Pyo; M Y Park; B K Ku; J Nah; S Ryoo; S H Wee; K S Choi; Haryoung Poo
Bibliographic Citation
Pathogens, vol. 8, pp. 301-301
Publication Year
2019
Abstract
Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A is one of the most common causes of FMD outbreaks among the seven FMDV serotypes, and its serological diagnosis is therefore important to confirm FMDV type A infection and to determine FMD vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific to FMDV type A via hybridoma systems using an inactivated FMDV type A (A22/Iraq/1964) and found 4 monoclones (#29, #106, #108, and #109) with high binding reactivity to FMDV type A among 594 primary clones. In particular, the #106 mAb had a higher binding reactivity to the inactivated FMDV type A than the other mAbs and a commercial mAb. Moreover, the #106 mAb showed no cross-reactivity to inactivated FMDV type South African territories 1, 2, and 3, and low reactivity to inactivated FMDV type O (O1 Manisa). Importantly, the solid-phase competitive ELISA (SPCE) using horseradish peroxidase (HRP)-conjugated #106 mAb detected FMDV type A-specific Abs in sera from FMD type A-vaccinated cattle more effectively than a commercial SPCE. These results suggest that the newly developed FMDV type A-specific mAb might be useful for diagnostic approaches for detecting Abs against FMDV type A.
Keyword
monoclonal antibodysensitivityfoot-and-mouth diseasesolid-phase competitive ELISAstructural proteintype A
ISSN
2076-0817
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/pathogens8040301
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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