Patterns of gene expression variation across body parts of the hydrothermal vent shrimp Nautilocaris saintlaurentae

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Title
Patterns of gene expression variation across body parts of the hydrothermal vent shrimp Nautilocaris saintlaurentae
Author(s)
R O Kim; E J Won; Hyun Mi Kang; S J Ju; Se Joo Kim
Bibliographic Citation
Ocean Science Journal, vol. 54, no. 4, pp. 595-609
Publication Year
2019
Abstract
Since deep-sea hydrothermal vents have been targeted for mining mineral resources, concerns about the potential impacts of mining on vent ecosystems have gradually increased. The vent alvinocaridid shrimp is one of the dominant endemic vent organisms. We collected an experimental species, Nautilocaris saintlaurentae, from the Tonga Arc in the Southwest Pacific Ocean, and firstly determined the sequences of seven housekeeping genes (HKGs) and three stress-related genes in this study. The stability of the HKGs was evaluated and the expression levels of targeted genes were measured in different body parts, the cephalothorax, abdomen, and whole body, to provide information on which parts of nonmodel species can be used for ecotoxicological studies. Based on geNorm platform, multiple combinations of HKGs were tested. The stability of the HKG expression in each body part indicated that the cephalothorax can be used alone and also demonstrated that using at least three HKGs provides suitable interpretations for mRNA real-time quantitative PCR arrays in N. saintlaurentae. Although analysis of specific body part subdivisions was not carried out, our results suggested that anatomical characteristics should be considered when investigating biological functions at a molecular level. Finally, this study revealed upregulation of stress-responsive genes in the cephalothorax of N. saintlaurentae with environmental changes.
Keyword
Alvinocarididaeglutathione peroxidaseheat shock proteinhousekeeping geneshydrothermal ventNautilocaris saintlaurentae
ISSN
1738-5261
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/s12601-019-0032-8
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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