Production of adipic acid by short- and long-chain fatty acid acyl-CoA oxidase engineered in yeast Candida tropicalis

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Title
Production of adipic acid by short- and long-chain fatty acid acyl-CoA oxidase engineered in yeast Candida tropicalis
Author(s)
Jung-Hyun Ju; Baek Rock OhSun-Yeon Heo; Young Uk Lee; Jung Hoon SohnChul Ho Kim; Y M Kim; Jeong-Woo Seo; W K Hong
Bibliographic Citation
Bioprocess and Biosystems Engineering, vol. 43, no. 1, pp. 33-43
Publication Year
2020
Abstract
In this study, to produce adipic acid, mutant strains of Candida tropicalis KCTC 7212 deficient of AOX genes encoding acyl-CoA oxidases which are important in the β-oxidation pathway were constructed. Production of adipic acid in the mutants from the most favorable substrate C12 methyl laurate was significantly increased. The highest level of production of adipic acid was obtained in the C. tropicalis ΔAOX4::AOX5 mutant of 339.8 mg L-1 which was about 5.4-fold higher level compared to the parent strain. The C. tropicalis ΔAOX4::AOX5 mutant was subjected to fed-batch fermentation at optimized conditions of agitation rate of 1000 rpm, pH 5.0 and methyl laurate of 3% (w/v), giving the maximum level of adipic acid of 12.1 g L-1 and production rate of 0.1 g L-1 h-1.
Keyword
Acyl-CoA oxidaseAdipic acidCandida tropicalisFed-batch fermentationMetabolic engineeringβ-Oxidation
ISSN
1615-7591
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00449-019-02202-w
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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