Identification of source-sink tissues in the leaf of Chinese cabbage (Brassica rapa ssp. pekinensis) by carbohydrate content and transcriptomic analysis

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Title
Identification of source-sink tissues in the leaf of Chinese cabbage (Brassica rapa ssp. pekinensis) by carbohydrate content and transcriptomic analysis
Author(s)
Jeongyeo Lee; X Dong; K Choi; H Song; H Yi; Y Hur
Bibliographic Citation
Genes & Genomics, vol. 42, no. 1, pp. 13-24
Publication Year
2020
Abstract
BACKGROUND: A leaf of Chinese cabbage (Brassica rapa ssp. pekinensis) is composed of a photosynthetic blade and a non-photosynthetic large midrib; thus each leaf contains both source and sink tissues. This structure suggests that, unlike in other plants, source-sink metabolism is present in a single leaf of Chinese cabbage. OBJECTIVE: This study was designed to identify the transport route of photosynthetic carbon and to determine whether both source and sink tissues were present in a leaf. METHODS: Plant samples were collected diurnally. Their carbohydrate contents were measured, and a genome-wide transcriptome analysis was performed using the Br300K microarray. Expression profiles of selected genes were validated using qRT-PCR analysis. RESULTS: The presence of two contrasting tissues (blade as source and midrib as sink) in a leaf was demonstrated by (1) diurnal distribution patterns of starch and sucrose content; (2) Gene Ontology (GO) enrichment analysis of microarray data; (3) expression profiles of photosynthetic and sucrose biosynthetic genes; and (4) expression patterns of a variety of sugar transporter genes. CONCLUSION: Source and sink tissues were both present in Chinese cabbage leaves, but the midrib functioned as a sink tissue as well as a site exporting to roots and other sink tissues. Function of most genes discriminating between source and sink tissue appeared to be regulated largely at the post-transcriptional level, not at the transcriptional level.
Keyword
Br300K microarrayCarbohydrate contentLarge midribPhotosynthetic genesSugar transporters
ISSN
1976-9571
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s13258-019-00873-z
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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