Increased O-GlcNAcylation of c-Myc promotes Pre-B cell proliferation

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dc.contributor.authorDa Hee Lee-
dc.contributor.authorNa Eun Kwon-
dc.contributor.authorW J Lee-
dc.contributor.authorMoo-Seung Lee-
dc.contributor.authorDoo-Jin Kim-
dc.contributor.authorJi Hyung Kim-
dc.contributor.authorSung-Kyun Park-
dc.date.accessioned2020-02-07T16:31:00Z-
dc.date.available2020-02-07T16:31:00Z-
dc.date.issued2020-
dc.identifier.issn20734409-
dc.identifier.uri10.3390/cells9010158ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19271-
dc.description.abstractO-linked β-N-acetylglucosamine (O-GlcNAc) modification regulates the activity of hundreds of nucleocytoplasmic proteins involved in a wide variety of cellular processes, such as gene expression, signaling, and cell growth; however, the mechanism underlying the regulation of B cell development and function by O-GlcNAcylation remains largely unknown. Here, we demonstrate that changes in cellular O-GlcNAc levels significantly affected the growth of pre-B cells, which rapidly proliferate to allow expansion of functional clones that express successfully rearranged heavy chains at the pro-B stage during early B cell development. In our study, the overall O-GlcNAc levels in these proliferative pre-B cells, which are linked to the glucose uptake rate, were highly induced when compared with those in pro-B cells. Thus, pharmacologically, genetically, or nutritionally, inhibition of O-GlcNAcylation in pre-B cells markedly downregulated c-Myc expression, resulting in cell cycle arrest via blockade of cyclin expression. Importantly, the population of B cells after the pro-B cell stage in mouse bone marrow was severely impaired by the administration of an O-GlcNAc inhibitor. These results strongly suggest that O-GlcNAcylation-dependent expression of c-Myc represents a new regulatory component of pre-B cell proliferation, as well as a potential therapeutic target for the treatment of pre-B cell-derived leukemia.-
dc.publisherMDPI-
dc.titleIncreased O-GlcNAcylation of c-Myc promotes Pre-B cell proliferation-
dc.title.alternativeIncreased O-GlcNAcylation of c-Myc promotes Pre-B cell proliferation-
dc.typeArticle-
dc.citation.titleCells-
dc.citation.number0-
dc.citation.endPage158-
dc.citation.startPage158-
dc.citation.volume9-
dc.contributor.affiliatedAuthorDa Hee Lee-
dc.contributor.affiliatedAuthorNa Eun Kwon-
dc.contributor.affiliatedAuthorMoo-Seung Lee-
dc.contributor.affiliatedAuthorDoo-Jin Kim-
dc.contributor.affiliatedAuthorJi Hyung Kim-
dc.contributor.affiliatedAuthorSung-Kyun Park-
dc.contributor.alternativeName이다희-
dc.contributor.alternativeName권나은-
dc.contributor.alternativeName이원지-
dc.contributor.alternativeName이무승-
dc.contributor.alternativeName김두진-
dc.contributor.alternativeName김지형-
dc.contributor.alternativeName박성균-
dc.identifier.bibliographicCitationCells, vol. 9, pp. 158-158-
dc.identifier.doi10.3390/cells9010158-
dc.subject.keywordO-GlcNAcylation-
dc.subject.keywordc-Myc-
dc.subject.keywordpre-B cell-
dc.subject.keywordproliferation-
dc.subject.localO-GlcNAcylation-
dc.subject.localc-Myc-
dc.subject.localpre-B cell-
dc.subject.localproliferation-
dc.subject.localProliferation-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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