Identification of a novel imprinted transcript in the porcine GNAS complex locus using methylome and transcriptome of parthenogenetic fetuses

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dc.contributor.authorJ Ahn-
dc.contributor.authorH Wu-
dc.contributor.authorJ Lee-
dc.contributor.authorI S Hwang-
dc.contributor.authorD Yu-
dc.contributor.authorJin Seop Ahn-
dc.contributor.authorJeong Woong Lee-
dc.contributor.authorS Hwang-
dc.contributor.authorK Lee-
dc.date.accessioned2020-02-07T16:31:04Z-
dc.date.available2020-02-07T16:31:04Z-
dc.date.issued2020-
dc.identifier.issn20734425-
dc.identifier.uri10.3390/genes11010096ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19286-
dc.description.abstractGenomic imprinting in domestic animals contributes to the variance of performance traits. However, research remains to be done on large-scale detection of epigenetic landscape of porcine imprinted loci including the GNAS complex locus. The purpose of this study was to generate porcine parthenogenetic fetuses and comprehensively identify imprinting patterns of the GNAS locus in transcript levels. To this end, both normally fertilized and bimaternal (uniparental) parthenogenetic porcine fetuses were generated, and whole genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-seq) were performed to construct methylome and transcriptome, respectively. Differentially methylated regions (DMRs) between the fetuses were identified through methylome analysis, and parental-origin-specific expression patterns of transcripts were examined with transcriptome. As a result, three major DMRs were identified: paternally methylated Nesp DMR, maternally methylated Nespas-Gnasxl DMR, and maternally methylated Exon1B-Exon1A DMR. Parental-origin-specific expressions of those five DMR-affected transcripts were found, including a novel imprinted transcript, Exon1B, in pigs. In conclusion, using parthenotes, parental-origin-specific imprinting patterns in the porcine GNAS locus was comprehensively identified, and our approach paves the way for the discovery of novel imprinted genes and loci in a genomic context across species.-
dc.publisherMDPI-
dc.titleIdentification of a novel imprinted transcript in the porcine GNAS complex locus using methylome and transcriptome of parthenogenetic fetuses-
dc.title.alternativeIdentification of a novel imprinted transcript in the porcine GNAS complex locus using methylome and transcriptome of parthenogenetic fetuses-
dc.typeArticle-
dc.citation.titleGenes-
dc.citation.number0-
dc.citation.endPage96-
dc.citation.startPage96-
dc.citation.volume11-
dc.contributor.affiliatedAuthorJin Seop Ahn-
dc.contributor.affiliatedAuthorJeong Woong Lee-
dc.contributor.alternativeName안진수-
dc.contributor.alternativeName우희광-
dc.contributor.alternativeName이준범-
dc.contributor.alternativeName황인설-
dc.contributor.alternativeNameYu-
dc.contributor.alternativeName안진섭-
dc.contributor.alternativeName이정웅-
dc.contributor.alternativeName황성수-
dc.contributor.alternativeName이기춘-
dc.identifier.bibliographicCitationGenes, vol. 11, pp. 96-96-
dc.identifier.doi10.3390/genes11010096-
dc.subject.keywordGNAS-
dc.subject.keywordRNA-sequencing-
dc.subject.keyworddifferentially methylated region-
dc.subject.keywordfetus-
dc.subject.keywordgenomic imprinting-
dc.subject.keywordparthenogenetic-
dc.subject.keywordparthenote-
dc.subject.keywordporcine-
dc.subject.keywordwhole genome bisulfite sequencing-
dc.subject.localGNAS-
dc.subject.localRNA-sequencing-
dc.subject.localdifferentially methylated region-
dc.subject.localDifferentially methylated region-
dc.subject.localfetus-
dc.subject.localgenomic imprinting-
dc.subject.localparthenogenetic-
dc.subject.localParthenogenetic-
dc.subject.localparthenote-
dc.subject.localporcine-
dc.subject.localPorcine-
dc.subject.localwhole genome bisulfite sequencing-
dc.subject.localwhole genome bisulfite sequencing (WGBS)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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