The velvet regulator VosA governs survival and secondary metabolism of sexual spores in Aspergillus nidulans

Cited 13 time in scopus
Metadata Downloads
Title
The velvet regulator VosA governs survival and secondary metabolism of sexual spores in Aspergillus nidulans
Author(s)
M J Kim; Mi Kyung Lee; H Q Pham; M J Gu; B Zhu; S H Son; D Hahn; J H Shin; J H Yu; H S Park; K H Han
Bibliographic Citation
Genes, vol. 11, pp. 103-103
Publication Year
2020
Abstract
The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans.
Keyword
Aspergillus nidulansVosAascosporessexual development
ISSN
2073-4425
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/genes11010103
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.