Engineering biology to construct microbial chassis for the production of difficult-to-express proteins = 발현이 어려운 단백질을 생산하기 위한 미생물 균주 개발

Cited 9 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorK Kim-
dc.contributor.authorD Choe-
dc.contributor.authorDae-Hee Lee-
dc.contributor.authorB K Cho-
dc.date.accessioned2020-04-24T16:30:12Z-
dc.date.available2020-04-24T16:30:12Z-
dc.date.issued2020-
dc.identifier.issn14220067-
dc.identifier.uri10.3390/ijms21030990ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19345-
dc.description.abstractA large proportion of the recombinant proteins manufactured today rely on microbe-based expression systems owing to their relatively simple and cost-effective production schemes. However, several issues in microbial protein expression, including formation of insoluble aggregates, low protein yield, and cell death are still highly recursive and tricky to optimize. These obstacles are usually rooted in the metabolic capacity of the expression host, limitation of cellular translational machineries, or genetic instability. To this end, several microbial strains having precisely designed genomes have been suggested as a way around the recurrent problems in recombinant protein expression. Already, a growing number of prokaryotic chassis strains have been genome-streamlined to attain superior cellular fitness, recombinant protein yield, and stability of the exogenous expression pathways. In this review, we outline challenges associated with heterologous protein expression, some examples of microbial chassis engineered for the production of recombinant proteins, and emerging tools to optimize the expression of heterologous proteins. In particular, we discuss the synthetic biology approaches to design and build and test genome-reduced microbial chassis that carry desirable characteristics for heterologous protein expression.-
dc.publisherMDPI-
dc.titleEngineering biology to construct microbial chassis for the production of difficult-to-express proteins = 발현이 어려운 단백질을 생산하기 위한 미생물 균주 개발-
dc.title.alternativeEngineering biology to construct microbial chassis for the production of difficult-to-express proteins-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Sciences-
dc.citation.number0-
dc.citation.endPage990-
dc.citation.startPage990-
dc.citation.volume21-
dc.contributor.affiliatedAuthorDae-Hee Lee-
dc.contributor.alternativeName김강산-
dc.contributor.alternativeName채동휘-
dc.contributor.alternativeName이대희-
dc.contributor.alternativeName조병관-
dc.identifier.bibliographicCitationInternational Journal of Molecular Sciences, vol. 21, pp. 990-990-
dc.identifier.doi10.3390/ijms21030990-
dc.subject.keyworddifficult-to-express proteins-
dc.subject.keywordgenome reduction-
dc.subject.keywordgenome synthesis.-
dc.subject.keywordheterologous protein expression-
dc.subject.keywordsynthetic biology-
dc.subject.keywordsystems biology-
dc.subject.localdifficult-to-express proteins-
dc.subject.localgenome reduction-
dc.subject.localGenome reduction-
dc.subject.localgenome synthesis.-
dc.subject.localheterologous protein expression-
dc.subject.localsynthetic biology-
dc.subject.localSynthetic biology-
dc.subject.localsystems biology-
dc.subject.localSystems biology-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.