Molecular cloning and functional characterization of a sweetpotato chloroplast IbDHAR3 gene in response to abiotic stress

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dc.contributor.authorW Wang-
dc.contributor.authorX Qiu-
dc.contributor.authorHo Soo Kim-
dc.contributor.authorY Yang-
dc.contributor.authorD Hou-
dc.contributor.authorX Liang-
dc.contributor.authorSang Soo Kwak-
dc.date.accessioned2020-04-24T16:30:14Z-
dc.date.available2020-04-24T16:30:14Z-
dc.date.issued2020-
dc.identifier.issn1863-5466-
dc.identifier.uri10.1007/s11816-019-00576-7ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19352-
dc.description.abstractDehydroascorbate reductase (DHAR) plays a critical role in the regeneration of ascorbic acid (AsA), and widely involved in plant tolerance to biotic and abiotic stresses. In this study, the IbDHAR3 gene was cloned from sweetpotato cultivar Xushu 18 by RT-PCR. The full-length of this gene was 813 bp which encodes 270 amino acids. The IbDHAR3 protein contained two conserved domains of glutathione S-transferase (GST) and GST-C-DHAR, and one chloroplast transit peptide with 52 amino acids length. Transient expression in tobacco leaf epidermal cells indicated that IbDHAR3 protein is subcellular localized to chloroplast. The qRT-PCR results revealed that the relative expression level of IbDHAR3 in leaves is much higher than that in other tissues, and could be up-regulated by ABA, drought, salinity, and high-temperature stresses. The seed germination rate and root elongation were increased in contrast to wild type under mannitol and NaCl stresses in T3 transgenic Arabidopsis overexpressing IbDHAR3 gene. The soil drought experiments showed that the overexpression of IbDHAR3 gene in Arabidopsis reduced the malondialdehyde (MDA) and the H2O2 content, enhanced the level of AtGR gene expression, superoxide dismutase (SOD), ascorbate peroxidase (APX), DHAR activity, and the AsA content. Therefore, overexpression of IbDHAR3 gene could enhance the ability of scavenging reactive oxygen species such as H2O2 by promoting AsA-glutathione cycle and related antioxidant enzymes system, thereby contributing to increased stress tolerance in Arabidopsis.-
dc.publisherSpringer-
dc.titleMolecular cloning and functional characterization of a sweetpotato chloroplast IbDHAR3 gene in response to abiotic stress-
dc.title.alternativeMolecular cloning and functional characterization of a sweetpotato chloroplast IbDHAR3 gene in response to abiotic stress-
dc.typeArticle-
dc.citation.titlePlant Biotechnology Reports-
dc.citation.number0-
dc.citation.endPage19-
dc.citation.startPage9-
dc.citation.volume14-
dc.contributor.affiliatedAuthorHo Soo Kim-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeNameWang-
dc.contributor.alternativeNameQiu-
dc.contributor.alternativeName김호수-
dc.contributor.alternativeNameYang-
dc.contributor.alternativeNameHou-
dc.contributor.alternativeNameLiang-
dc.contributor.alternativeName곽상수-
dc.identifier.bibliographicCitationPlant Biotechnology Reports, vol. 14, pp. 9-19-
dc.identifier.doi10.1007/s11816-019-00576-7-
dc.subject.keywordAscorbic acid-
dc.subject.keywordFunctional characterization-
dc.subject.keywordIbDHAR3-
dc.subject.keywordStress tolerance-
dc.subject.keywordSweetpotato-
dc.subject.localascorbic acid-
dc.subject.localAscorbic acid-
dc.subject.localAscorbic acid (vitamin C)-
dc.subject.localFunctional characterization-
dc.subject.localIbDHAR3-
dc.subject.localstress tolerance-
dc.subject.localStress tolerance-
dc.subject.localsweet potato-
dc.subject.localsweet potatoes-
dc.subject.localSweet potato (Ipomoea batatas L. Lam)-
dc.subject.localSweet potato (Ipomoea batatas)-
dc.subject.localSweet otato-
dc.subject.localsweet potato (Ipomoea batatas)-
dc.subject.localipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas)-
dc.subject.localsweetpotato-
dc.subject.localSweetpotato Ipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas L.)-
dc.subject.localSweetpotato-
dc.subject.localSweetpotato (Ipomoea batatas (L.) Lam)-
dc.subject.localSweet potato-
dc.subject.localIpomoea batatas-
dc.subject.localSweet potato (Ipomoea batatas (L.) Lam.)-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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