Regulation of lipid accumulation using nitrogen for microalgae lipid production in Schizochytrium sp. ABC101

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Title
Regulation of lipid accumulation using nitrogen for microalgae lipid production in Schizochytrium sp. ABC101
Author(s)
Jung-Hyun Ju; Dong-Jin Ko; Sun-Yeon Heo; Jong-Jea Lee; Y M Kim; B S Lee; Min-Soo KimChul Ho KimJeong-Woo SeoBaek Rock Oh
Bibliographic Citation
Renewable Energy, vol. 153, pp. 580-587
Publication Year
2020
Abstract
Lipid-rich microalgae are a potential source for the bio-oil production. We isolated a novel oleaginous Thraustochytrid microalga from seawater, Schizochytrium sp. ABC101, and tested the effect of different growth conditions on its production of lipids. Batch fermentation experiments indicated that depletion of nitrogen led to lipid accumulation, and lipid accumulation led to increased cell size and decreased cell wall thickness. Based on these results, we tested three types of fed-batch fermentations (carbon and no nitrogen feeding, carbon and limited nitrogen feeding, carbon and sufficient nitrogen feeding) to examine the effect of adjusting the nitrogen supply on lipid accumulation. The limited nitrogen feeding provided the most efficient lipid production, with maxima after 60 h (dry cell weight [DCW]: 64.7 g/L, lipids: 25.4 g/L, docosahexaenoic acid [DHA]: 11.0 g/L). Use of corn steep liquor (CSL) as the sole nitrogen source also led to excellent performance, with maxima at 84 h (DCW: 86.0 g/L, lipids: 37.2 g/L, DHA: 16.7 g/L). In addition, use of CSL rather than yeast extract reduced the lipid production cost by 50%. Our results suggest that regulation of the nitrogen source improves the efficiency of microalgal lipid production and use of a low-cost nitrogen source reduces production costs.
Keyword
BiodieselCorn steep liquorDocosahexaenoic acidLipid regulationMicroalgal bio-oilNitrogen regulation
ISSN
0960-1481
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.renene.2020.02.047
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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