Roles of protein histidine phosphatase 1 (PHPT1) in brown adipocyte differentiation

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Title
Roles of protein histidine phosphatase 1 (PHPT1) in brown adipocyte differentiation
Author(s)
Joo Ae Kang; Hyun Sup Kang; Kwang-Hee BaeSang Chul LeeKyoung-Jin OhWon Kon Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 30, no. 2, pp. 306-312
Publication Year
2020
Abstract
Despite the importance of brown adipocytes as a therapeutic target for the prevention and treatment of obesity, the molecular mechanism underlying brown adipocyte differentiation is not fully understood. In particular, the role of post-translational modifications in brown adipocyte differentiation has not been extensively studied. Histidine phosphorylation is increasingly recognized an important process for protein post-translational modifications. In this study, we show that histidine phosphorylation patterns change during brown adipocyte differentiation. In addition, the expression level of protein histidine phosphatase 1 (PHPT1), a major mammalian phosphohistidine phosphatase, is reduced rapidly at the early phase of differentiation and recovers at the later phase. During white adipocyte differentiation of 3T3- L1 preadipocytes, however, the expression level of PHPT1 do not significantly change. Knockdown of PHPT1 promotes brown adipocyte differentiation, whereas ectopic expression of PHPT1 suppresses brown adipocyte differentiation. These results collectively suggest that histidine phosphorylation is closely linked to brown adipocyte differentiation and could be a therapeutic target for obesity and related metabolic diseases.
Keyword
Brown adipogenesisPHPT1histidine phosphorylationobesity
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1909.09003
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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