Comprehensive DNA methylation profiling identifies novel diagnostic biomarkers for thyroid cancer

Cited 19 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJong Lyul Park-
dc.contributor.authorS Jeon-
dc.contributor.authorEun Hye Seo-
dc.contributor.authorDong Hyuck Bae-
dc.contributor.authorY M Jeong-
dc.contributor.authorY Kim-
dc.contributor.authorJ S Bae-
dc.contributor.authorSeon-Kyu Kim-
dc.contributor.authorC K Jung-
dc.contributor.authorYong Sung Kim-
dc.date.accessioned2020-04-24T16:30:25Z-
dc.date.available2020-04-24T16:30:25Z-
dc.date.issued2020-
dc.identifier.issn1050-7256-
dc.identifier.uri10.1089/thy.2019.0011ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/19397-
dc.description.abstractBackground: There are no reliable biomarkers to accurately differentiate indolent thyroid tumors from more aggressive thyroid cancers. This study aimed to develop new DNA methylation markers for diagnosis and recurrence risk stratification of papillary thyroid carcinoma (PTC). Methods: Thyroid tumor-specific DNA methylation profiling was investigated in 34 fresh frozen tissues, which included nontumor (n=7), noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP, n=6) and PTC (n=21), using the Illumina HumanMethylation EPIC array. We performed a genome-wide assessment of thyroid tumor-specific differentially methylated CpG sites in the discovery set, then validated the top candidate markers in an independent set of 293 paraffin tissue samples comprised of follicular adenoma (FA, n=61), Hurthle cell adenoma (HA, n=24), NIFTP (n=56), PTC (n=120), follicular thyroid carcinoma (n=27), and Hurthle cell carcinoma (n=5), by pyrosequencing. Results: Three selected markers (cg10705422, cg17707274, and cg26849382) differentiated nonmalignant (FA, HA, and NIFTP) tumors from differentiated thyroid cancers with area under the receiver operating characteristic curve of 0.83, 0.83, and 0.80, respectively. Low DNA methylation levels for three markers were significantly associated with recurrent or persistent disease (odds ratio (OR)=3.860 [95% confidence interval (CI) 1.194-12.475]) and distant metastasis (OR=4.009 [CI 1.098-14.632]) in patients with differentiated thyroid cancer. A subgroup analysis for the validation set showed that PTC patients with low DNA methylation levels more frequently had aggressive histology, extrathyroidal extension, lymph node metastasis, BRAFV600E mutations, and recurrent or persistent disease than those with high levels of methylation markers. All PTC patients who developed disease recurrence had low DNA methylation levels for three markers. Conclusions: DNA methylation levels of three markers can be useful for differentiating differentiated thyroid cancer from nonmalignant follicular thyroid lesions, and may serve as prognostic biomarkers for predicting recurrent or persistent disease after surgery for differentiated thyroid cancer.-
dc.publisherMary Ann Liebert, Inc-
dc.titleComprehensive DNA methylation profiling identifies novel diagnostic biomarkers for thyroid cancer-
dc.title.alternativeComprehensive DNA methylation profiling identifies novel diagnostic biomarkers for thyroid cancer-
dc.typeArticle-
dc.citation.titleThyroid-
dc.citation.number2-
dc.citation.endPage203-
dc.citation.startPage192-
dc.citation.volume30-
dc.contributor.affiliatedAuthorJong Lyul Park-
dc.contributor.affiliatedAuthorEun Hye Seo-
dc.contributor.affiliatedAuthorDong Hyuck Bae-
dc.contributor.affiliatedAuthorSeon-Kyu Kim-
dc.contributor.affiliatedAuthorYong Sung Kim-
dc.contributor.alternativeName박종열-
dc.contributor.alternativeName전소라-
dc.contributor.alternativeName서은혜-
dc.contributor.alternativeName배동혁-
dc.contributor.alternativeName정영문-
dc.contributor.alternativeName김유라-
dc.contributor.alternativeName배재성-
dc.contributor.alternativeName김선규-
dc.contributor.alternativeName정찬권-
dc.contributor.alternativeName김용성-
dc.identifier.bibliographicCitationThyroid, vol. 30, no. 2, pp. 192-203-
dc.identifier.doi10.1089/thy.2019.0011-
dc.subject.keywordDNA methylation-
dc.subject.keywordNIFTP-
dc.subject.keywordmethylation markers-
dc.subject.keywordpapillary thyroid cancer-
dc.subject.keywordrecurrence-
dc.subject.keywordthyroid neoplasms-
dc.subject.localDNA methylation-
dc.subject.localDNAmethylation-
dc.subject.localNIFTP-
dc.subject.localmethylation markers-
dc.subject.localmethylation marker-
dc.subject.localpapillary thyroid cancer-
dc.subject.localrecurrence-
dc.subject.localRecurrence-
dc.subject.localThyroid neoplasms-
dc.subject.localthyroid neoplasms-
dc.description.journalClassY-
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.