Staphylococcus aureus specific FRET probe-based antibacterial susceptibility testing (SF-AST) by detection of micrococcal nuclease activity

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Title
Staphylococcus aureus specific FRET probe-based antibacterial susceptibility testing (SF-AST) by detection of micrococcal nuclease activity
Author(s)
S Y Yi; Jinyoung Jeong; K E Kim; K Park; Yong Beom Shin
Bibliographic Citation
ACS Infectious Diseases, vol. 6, no. 2, pp. 215-223
Publication Year
2020
Abstract
In this study, we describe a simple and rapid antibacterial susceptibility testing (AST) method for Staphylococcus aureus called S. aureus specific fluorescence resonance energy transfer (FRET) probe-based AST (SF-AST), which is based on an S. aureus specific FRET probe (SF probe) that detects micrococcal nuclease (MNase) activity secreted from S. aureus. The SF-AST was tested with an S. aureus quality control (QC) strain against six relevant antibiotics, and the minimum inhibitory concentration (MIC) values obtained with the broth microdilution (BMD) method were compared, as a gold standard AST. Results were obtained with high accuracy in 4-6 h. The MIC for the methicillin resistance using 20 clinical S. aureus isolates of SF-AST showed 100% sensitivity, specificity, positive predictive value, and negative predictive value, as compared to BMD. Thus, the SF-AST method is a simple, rapid, and useful antibiotic resistance test for S. aureus, and it provides a basis for clinical treatment in a short time.
Keyword
antibacterial susceptibility testingStaphylococcus aureusfluorescence resonance energy transfermicrococcal nuclease
ISSN
2373-8227
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/acsinfecdis.9b00260
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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