Ar-gas cluster ion beam in ToF-SIMS for peptide and protein analysis

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Ar-gas cluster ion beam in ToF-SIMS for peptide and protein analysis
J G Son; S Yoon; H K Shon; Jeong Hee Moon; S Joh; T G Lee
Bibliographic Citation
Biointerphases, vol. 15, no. 2, pp. 021011-021011
Publication Year
Since Ar-gas cluster ion beams (Ar-GCIBs) have been introduced into time-of-flight secondary ion mass spectrometry (ToF-SIMS), there have been various attempts to analyze organic materials and biomolecules that require low-damage analysis and high sensitivity, because Ar-GCIBs allow soft ionization of large molecules such as peptides and proteins due to the low energy per atom. Here, the authors adopted the Ar-GCIB as a primary beam to detect proteins including human insulin, ubiquitin, and cytochrome C (molecular weights are 5808, 8564, and 12 327 Da, respectively). They have confirmed that the detection of the intact proteins was possible when the Ar-GCIB was used as a primary ion beam. In addition, they successfully identified each protein by analyzing the trypsin-digested peptides in myoglobin, cytochrome C, and bovine serum albumin. They also attempted on-surface enzymatic digestion to identify proteins on the surface of the Si wafer and obtained results identical to those of in-solution digestion. It is expected that the authors' on-surface digestion method can enable the application of ToF-SIMS for the analysis of proteins present in biological tissues.
Amer Inst Phys
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Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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